摘要
目的探讨表没食子儿茶素没食子酸酯(EGCG)干预抗β2GPI/β2GPI复合物诱导人急性单核细胞白血病细胞系(THP-1细胞)活化的作用。方法用一定剂量的EGCG、脂多糖(LPS)、抗β2GPI/β2GPI复合物等不同刺激物处理THP-1细胞,实时荧光定量PCR检测细胞组织因子(TF)及肿瘤坏死因子-α(TNF-α)mRNA表达;发色底物法检测TF活性;ELISA测定TNF-α蛋白的表达。结果 50 mg/L EGCG能抑制抗β2GPI/β2GPI复合物刺激的THP-1细胞TF mRNA和活性的表达(t值为6.97、3.89,P均<0.05),同时下调该复合物诱导的THP-1细胞TNF-αmRNA和蛋白质的表达水平(t值为3.23、4.65,P均<0.05)。结论 EGCG可干预抗β2GPI/β2GPI复合物诱导THP-1细胞TF及TNF-α的表达,抑制THP-1细胞活化。
Objective To explore the interference effects of epigallocatechin-3-gallate （EGCG） on anti-β2GPI/β2GPI complex-induced activation of human acute nmnocytic leukemia cell line THP-1. Methods In vitro, THP-1 cells were treated with EGCG, lipopolysaccharide （LPS） and anti-β2GPI/β2GPI complex. The mRNA expressions of tissue factor （TF） and TNF-α in THP-1 cells were detected by real-time quantitative PCR. The activity of TF was determined by chromogenic assay. TNF-α protein in the cell culture supernatant was measured by ELISA. Results EGCG （50 mg/L） could reduce TF mRNA expression and TF activity in THP-1 cells stimulated by anti-β2GPI/β2GPI complex （t = 6. 97,3.89 ,P 〈 0.05 ）, and also decrease the level of both mRNA and protein of TNF-α in THP-1 cells induced by anti-β2GPI/β2GPI complex （t = 3.23, 4.65,P 〈 0.05 ）. Conclusion EGCG could intercept the effects of anti-β2GPI/β2GPI complex on the activation of THP-1 cells by decreasing the expression of TF and TNF-α.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2013年第6期430-433,共4页
Chinese Journal of Clinical Laboratory Science
基金
国家自然科学基金(30971301)
江苏大学博士创新基金(CX08B_16x)
