摘要
通过牛血清白蛋白(BSA)辅助交联,制备了牛血清白蛋白苯丙氨酸解氨酶交联酶聚体(BSA-PAL-CLEAs)。优化了BSA-PAL-CLEAs的制备条件,并比较研究了游离酶、苯丙氨酸解氨酶交联酶聚体(PAL-CLEAs)和BSA-PAL-CLEAs的稳定性差异。研究发现,BSA-PAL-CLEAs的最佳制备条件:BSA浓度为5mg/mL,硫酸铵饱和度80%,戊二醛(10%v/v)10μL,交联时间2h,所得BSA-PAL-CLEAs的酶活回收率(25%)是不加BSA时(13%)的1.92倍。稳定性研究表明,BSA-PAL-CLEAs的温度稳定性、储藏稳定性比PAL-CLEAs都有所提高;但是,游离酶、PAL-CLEAs、BSA-PAL-CLEAs的pH稳定性相差不大;BSA-PAL-CLEAs在有机溶剂中的稳定性比PAL-CLEAs有所下降。
Cross-linked enzyme aggregates of phenylalanine ammonia lyase（BSA-PAL-CLEAs） were prepared by using BSA as an additive.Conditions for the preparation of BSA-PAL-CLEAs were optimized, and the stability of free PAL,PAL-CLEAs and BSA-PAL-CLEAs was also evaluated, respectively.The optimized conditions of BSA- PAL-CLEAs preparation were as follows.80%-saturation ammonia sulfate,5mg/mL BSA, 1μ0L glutaraldehyde （10% v/v）and 2h cross-linking time. Under optimized conditions, the activity recovery of prepared BSA-PAL- CLEAs was 1.92-fold than that of PAL-CLEAs.Moreover,the thermal stability and storage stability of BSA-PAL- CLEAs was higher than that of PAL-CLEAs.However, stability against pH of free PAL, PAL-CLEAs and BSA-PAL- CLEAs was similar. In addition, compared with PAL- CLEAs, the stability of BSA- PAL- CLEAs against organic solvents was decreased.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第13期66-69,74,共5页
Science and Technology of Food Industry
基金
国家自然科学基金(21072041)资助
关键词
牛血清白蛋白
苯丙氨酸解氨酶
交联酶聚体
bovine serum albumin
phenylalanine ammonia lyase
cross-linked enzyme aggregates
