摘要
目的通过探讨Spry1和MAPK蛋白在病毒性心肌炎(viral myocarditis,VMC)心肌组织中的表达,揭示其发病及猝死机制,并为法医学心源性猝死的鉴定提供指导。方法 30只Balb/c雄性小鼠随机分为VMC组和对照组,分别腹腔注射柯萨奇B3病毒和Eagel’s培养液,处死小鼠取心脏,进行常规病理检查,并通过免疫组织化学、Western印迹法及real-time PCR方法检测心肌组织中Spry1蛋白及mRNA、MAPK蛋白表达变化。结果 VMC组在光镜下可见心肌间质水肿,广泛炎症细胞浸润,心肌细胞坏死,心肌纤维形成多处灶性、大片状坏死,心肌组织中Spry1蛋白表达水平低于对照组(P<0.05),Spry1 mRNA水平略降低(P>0.05);而MAPK蛋白表达水平在VMC组高于对照组(P<0.05)。结论 Spry1参与的MAPK/ERK通路在病毒性心肌炎心肌纤维化过程中起着促进胶原表达的作用,从而导致心律失常、心功能减退甚至心源性猝死。
Objective To discuss the myocardial expression of Spryl and MAPK proteins of viral my ocarditis （VMC）, to reveal its mechanism of sudden death, and to provide guides for forensic identifica tion of sudden cardiac death. Methods Thirty Balb/c male mice were randomly divided into VMC group and control group, inoculated intraperitoneally with Coxsackievirus B3 and Eagel＇s solution, respective ly. After the mice were sacrificed, the cardiac tissues of the mice were taken to proceed regular patho logical examination. The changes of Spryl protein, Spryl mRNA and MAPK protein were detected by immunohistochemistry, Western blotting and realtime PCR. Results Under light microscope, the patho logic changes included myocardial interstitial edema, inflammatory cells infiltration, myocardial necrosis, and focal and patchy necrosis of myocardial fiber in VMC group. The expression of Spryl protein in VMC group was lower than that in control group （P〈0.05）. There was slightly decreased expression of Spryl of the mRNA level in VMC group （P〉0.05）. But the MAPK protein expression in VMC group was high er than that in control group （P〈0.05）. Conclusion The pathway of MAPK/ERK involving Spryl protein accelerates the expression of collagen, which may contribute to arrhythmia, heart failure and even sud den cardiac death.
出处
《法医学杂志》
CAS
CSCD
2013年第3期164-167,共4页
Journal of Forensic Medicine
基金
国家自然科学基金资助项目(81172897)
南通市自然科学基金资助项目(BK2011053)
