摘要
目的通过检测C35蛋白在乳腺癌及正常乳腺组织中的表达,揭示C35蛋白在乳腺癌发生、发展、侵袭转移过程中的作用和意义。方法收集68例女性乳腺癌组织及20例乳腺正常组织,应用免疫组化技术检测分析C35蛋白在乳腺癌组织中的表达及其与乳腺癌临床病理特征的关系;分析乳腺癌组织C35蛋白表达与ER、PR、HER-2蛋白表达的相关性及与肿瘤不同分子亚型的关系。结果 1)乳腺癌组织中C35蛋白的表达显著高于正常乳腺组织(P<0.05),而在正常乳腺组织C35蛋白呈现低表达或不表达。C35蛋白表达与乳腺癌组织临床病理学特点存在相关性,随着乳腺癌组织的临床TNM分期、病理组织学分级的增加,C35蛋白阳性率显著升高(P<0.05);2)乳腺癌组织C35蛋白表达与ER与PR的表达呈负相关(r分别为-0.035和-0.083),与HER-2蛋白的表达呈正相关(r=0.207);3)C35在乳腺癌不同分子亚型中的阳性率差异无统计学意义(P>0.05),有无淋巴结转移者C35蛋白阳性率差异有统计学意义(P<0.05)。结论 C35蛋白在乳腺癌细胞中广泛存在,并与乳腺癌的发生、发展及预后有关,因而可作为乳腺癌诊断和治疗的标志物或新靶点。
Objective To detect the expression of C35 protein in breast cancer and normal breast tissues, reveal the occurrence, C35 protein in breast cancer development, invasion and meaning transfer process. Methods 68 cases of breast carcinoma and 20 cases of normal breast tissue, immunohistochemical staining was used to detect the expression of C35 protein in breast cancer tissues and its relationship with the clinical pathological characteristics of breast cancer; analysis of the relationship between the expression of C35 protein in breast cancer tissues and ER, PR, HER-2 protein expression and its correlation with tumor molecular subtypes. Results 1) the expression of C35 protein in breast cancer tissues was significantly higher than that in normal breast tissue (P〈 0. 05), and in the C35 protein in normal breast tissue showed low expression or no expression. Correlation characteristics and clinical pathology in breast cancer tissue C35 pro tein expression, with the increase of clinical TNM in breast cancer staging, histological grade, positive rate of C35 protein was significantly increased (P〈 0.05) ; 2) C35 protein in breast cancer tissue expression was negatively correlated with the expression of ER and PR (R respectively for -0. 035 and -0. 083), and HER-2 expression was positively related to protein (r=0. 207); 3) had no significant difference between the positive rate of C35 in breast cancer with different molecular subtypes of (P〈 0.05), there is no node difference in positive rate of C35 protein in lymph metastasis were statistically significant (P〈 0.05). Conclusion C35 protein existed in breast cancer cells, and the occurrence, development and prognosis associated with breast cancer, which may be used as a marker of diagnosis and treatment of breast cancer and a new target.
出处
《中国病原生物学杂志》
CSCD
北大核心
2013年第6期551-555,共5页
Journal of Pathogen Biology
基金
国家自然科学基金项目(No.81041075)
关键词
乳腺癌
C35蛋白
表达
免疫组化技术
Breast cancer
C35 protein
expression
immunohistochemistry