摘要
目的研究临床常用8种抗菌药物对嗜麦芽窄食单胞菌(Stenotrophomonas maltophilia,SMA)体外生物被膜(bacterial biofilm,BBF)的抗菌活性。方法通过微量接种针装置建立SMA生物被膜的体外模型,测定左氧氟沙星、环丙沙星、哌拉西林、头孢哌酮/舒巴坦、头孢他啶、磺胺甲基异噁唑、庆大霉素和红霉素对SMA生物被膜的抑制浓度(biofilm inhibitory concentration,BIC),并与相应最低抑菌浓度(minimum inhibitory concentration,MIC)进行比较。结果共有42株SMA利用微量接种针成功建立成熟生物被膜的体外模型,8种抗菌药物对形成物被膜SMA的BIC50分别为左氧氟沙星4μg/ml、环丙沙星8μg/ml、哌拉西林256μg/ml、头孢哌酮/舒巴坦128μg/ml、头孢他啶128μg/ml、磺胺甲基异噁唑304μg/ml、庆大霉素256μg/ml、红霉素128μg/ml,相应抗生素的MIC50分别为0.25、2、64、16、32、19、32和32μg/ml。相应的BIC90也均高于MIC90。扫描电镜观察细菌培养24h可形成成熟的生物被膜形态结构。结论与浮游态细菌相比,形成生物被膜后SMA对抗菌药物的耐药程度增加。
Objective To investigate the in vitro effects of eight antibiotic agents on Stenotrophomonas maltophilia bio- films. Methods An in vitro model of S. maltophilia biofilms was established in a Mueller-Hinton broth-silica film system, the biofilm inhibitory concentration （BIC） of each agent was calculated, and the corresponding minimal inhibitory concentrations （MICs） of the agents were compared. Results The in vitro model of S. maltophilia biofilms was subjected to scanning electron microscopy. Forty-two strains formed bacterial biofilms. The BIC50of levofloxacin, ciprofloxacin, piperacillin, cefoperazone/sulbactam, ceftazidime, sulfamethoxazole, gentamyein, and erythromyein was 4μg/ml, 8μg/ml, 256 μg/ml, 128μg/ml, 128μg/ml, 304μg/ml, 256μg/ml, and 128μg/ml, respectively. The corresponding of MIC50 of these agents was 0.25 μg/ml, 2 μg/ml, 64 μg/ml, 16 μg/ml, 32 μg/ml, 19 μg/ml, 32 μg/ml, and 32 μg/ml, respectively. Similarly, the BIC90 of each agent was much higher than the corresponding MIC after biofilms were formed. Conclusion Compared to planktonic phase growth, after the formation of biofilm S. maltophilia was highly resistant to most antimicrobial agents.
出处
《中国病原生物学杂志》
CSCD
北大核心
2013年第6期497-500,共4页
Journal of Pathogen Biology
基金
天津市高等学校科技发展基金项目(20050210)
关键词
嗜麦芽窄食单胞菌
细菌生物被膜
药敏试验
抑菌浓度
Stenotrophomonas maltophilia
bacterial biofilm
susceptibility testing
inhibitory concentration
