摘要
运用吖啶橙检测法研究中华鳖 (Trionyxsinensis)嗜中性粒细胞吞噬功能。试验测定了中华鳖嗜中性粒细胞对金黄色葡萄球菌 (Staphylococcusaureus)和大肠杆菌 (Escherichiacoil)的吞噬和杀伤能力。结果发现 ,中华鳖嗜中性粒细胞分别和金黄色葡萄球菌、大肠杆菌温育 30～ 180min ,吞噬百分率分别由 15%增加到 6 5%、由 9%增加到 6 3% ,杀伤百分率由 6 %增加到 6 2 %、由 2 %增加到 6 4 % ,吞噬指数由 0 .56增加到11.92、由 0 .14增加到 10 .77,杀伤指数由 0 .32增加到 0 .95、由 0 .2 0增加到 0 .95。温育 180min以内 ,中华鳖嗜中性粒细胞对 2种细菌的吞噬和杀伤能力随着时间的延长而增强 ,并且对金黄色葡萄球菌的吞噬和杀伤能力强于对大肠杆菌的。 180min以后 ,中华鳖嗜中性粒细胞对 2种细菌的吞噬和杀伤能力达到最大值 ,两者的数值相近。 2 4
Acridine orange (AO) method was employed to measure the phagocytic activity of neutrophilic granulocyte in Trionyx sinensis. The method could be used to assay both phagocytosis and bactericidal function simultaneously through fluorescence microscopy. The results show that when incubated in medium temperature of 26.7℃ for 30～240 min with Staphylococcus aureus and Escherichia ciol, respectively, the neutrophilic granulocytes increased from 15% to 65% (at 180th minute, the same below)and 9% to 63%; the percent killing increased from 6% to 62% and 2% to 64%; the phagocytic index increased from 0.56 to 11.92 and 0.14 to 10.77, respectively, and the killing indexes increased from 0.32 to 0.95 and from 0.20 to 0.95, respectively. Within 180 min of the incubation, the phagocytic and bactericidal capacity of Trionyx sinensis neutrophilic granulocytes increased with time and the phagocytic and bactericidal capacity of neutroophilic granulocytes to Saphylococcus aureus was larger than to Escherichia coli. At 180 th minute, the phagocytic and bactericidal capacity of neutrophilic granulocytes to the 2 strains reached the peak. There was little difference between them. At 240 th minute all the values decreased.
出处
《中国水产科学》
CAS
CSCD
2000年第2期32-36,共5页
Journal of Fishery Sciences of China
基金
国家自然科学基金资助项目! ( 3 9970 5 82 )
上海市教委重点科学基金部分资助 !(科 97-3 1)
关键词
中华鳖
嗜中性粒细胞
吞噬功能
吖啶橙检测法
Trionyx sinensis
neutrophilic granulocyte
phagocytosis, acridine orange method
