摘要
建立了饮料中10种人工着色剂(柠檬黄、日落黄、胭脂红、苋菜红、诱惑红、赤藓红、亮蓝、新红、酸性红、罗丹明B)的高效液相色谱测定方法。样品调pH值后,采用Welch Ultimate XB-C18色谱柱(4.6mm×150mm,5μm),以0.02mol/L乙酸铵和甲醇梯度洗脱,采用紫外检测器检测。10种人工合成色素在0.8-40mg/L范围内线性良好,相关系数(r)都在0.999以上,方法加标回收率为90.3-108.0%。该方法简便,易操作,灵敏度高,分离度好,适用于饮料中10种着色剂的同时测定。
A high performance liquid chromatography （HPLC） methods for determination of 10 synthetic colours （tartrazine, sunset yellow, carmine , amaranth, allura red, erythrosine, brilliant blue, new red, azorubine, Rho-damine B） in drinks was developed. The sample pH value was adjusted and then HPLC analysis was performed on pretreated samples. The pigments were separated on a Welch Ultimate XB-C18 column （51~m, 4.6mmx250mm） by gradient elution with a mobile phases made up of 0.02rnol/L NH4Ac and methanol, and detected by a UV detector. The ten kinds of synthetic colours had good linearity in the range of 0.8-40mg/L, the correlation coefficient （r） was higher than 0.999, and method recovery was 92.0-108.0%. This method was simple, easy to operate, the results were also good, accurate and reliable. This method has met the requirement of analysis method of 10 synthetic colours.
出处
《食品与发酵科技》
CAS
2013年第3期68-71,共4页
Food and Fermentation Science & Technology
基金
重庆市质量技术监督局科技项目(KY201103)
关键词
高效液相色谱法
着色剂
饮料
high performance liquid chromatography
synthetic colours
drinks
