摘要
来源于水母Aequoreavictoria的绿色荧光蛋白 ( greenfluorescentprotein ,GFP)现已成为在生物化学和细胞生物学中研究和开发应用得最广泛的蛋白质之一 .其内源荧光基团在受到紫外光或蓝光激发时 (λmax=395nm ,小峰在 4 79nm )可高效发射清晰可见的绿光 .GFP的高分辨率晶体结构为了解和研究蛋白质结构和光谱学功能关系提供了一个极好的机会 .GFP已成为一个监测在完整细胞和组织内基因表达和蛋白质定位的理想标记 .通过突变和蛋白质工程构建的GFP嵌合蛋白在生理指示剂、生物传感器。
Recently the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has emerged . It has been widely studied and used in biochemistry and cell biology. The protein emits green light ( λ max =508 nm) when excited with ultraviolet (UV) or blue light ( λ max =395 nm, minor peak at 479 nm). It is extremely useful as a marker for monitoring gene expression and as a tag in studying protein localization in organisms, intact cells and organelle. Mutagenesis and engineering of GFP fused into chimeric proteins are opening new prospect in physiological indicators, biosensors, photo chemistry and production of luminescent fiber.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2000年第3期238-243,共6页
Progress In Biochemistry and Biophysics
