摘要
结合花药培养和常规选育 ,从 77_5 433×中 5杂交组合后代中选育出 6个抗大麦黄矮病毒 (barleyyellowdwarfvirus ,BYDV)、染色体数目为 42的小麦 (TriticumaestivumL .)新种质 ,并用减数分裂配对分析、单体小麦测交法、基因组原位杂交、C分带、同工酶等电聚焦和RAPD对上述材料进行了综合鉴定 ;表明这些材料都是小麦_中间偃麦草(AgropyronintermediumGarten)二体异代换系 ,其抗病性来自其携带的一对中间偃麦草染色体 (来源于中 5 )。
By the method of combined anther culture and conventional selection, six new BYDV (barley yellow dwarf virus)_resistant wheat germplasms (2n=42) were obtained from the crosses between common wheat cv. 77_5433 and Zhong 5, a partial Triticum aestivum_Agropyron intermedium amphiploid. The six germplasms were comprehensively identified by meiosis pairing analysis, testing cross analysis using monosomic analysis, C_banding, isoelectrofocusing (IEF) of isoenzyme and RAPD analyses. The results indicated that all the germplasms were alien substitution lines carrying resistant genes originated from Zhong 5.
基金
国家自然科学基金资助项目!( 3 9770 5 17)&&
关键词
BYDV
抗病种质
二体异代换系
小麦
抗性鉴定
Zhong 5
BYDV_resistant germplasms
alien disomic substitution lines
cytological
biochemical
and molecular identification
