摘要
目的:观察国产甲型H1N1流感病毒亚单位疫苗对小鼠的免疫原性。方法:将不同血凝素含量(20、30、40μg/ml)各3批甲型H1N1流感病毒亚单位疫苗分别免疫小鼠,腹腔注射0.5 ml/只,每批疫苗又分为1针组、2针组,每组10只,2针间隔1周,同时设对照组。首针免疫后21天,分别采血,分离血清,检测血凝抑制抗体效价和中和抗体效价,观察疫苗的免疫原性。结果:血凝素含量为20μg/ml的甲型H1N1流感病毒亚单位疫苗1针组、2针组血凝抑制抗体效价均小于1∶40,中和抗体效价均小于1∶200;血凝素含量为30、40μg/ml的甲型H1N1流感病毒亚单位疫苗1针组、2针组的血凝抑制抗体效价均大于1∶40,中和抗体效价均大于1∶200;血凝素含量为30、40μg/ml的甲型H1N1流感病毒亚单位疫苗1针组和2针组的血凝抑制抗体和中和抗体效价比较,差异均无统计学意义。结论:血凝素含量为30μg/ml的甲型H1N1流感病毒亚单位疫苗接种1针即可达到理想的免疫效果。
Objective： To observe the immunogenicity of domestic H1N1 influenza subunit vaccine in mice. Methods： The H1 N1 influenza subunit vaccines with various hemagglutinin contents （20, 30, 40μg/ml）, three batches for each, were used for im- munization of mice. Mice were divided various groups, 10 for each, and were injected ip. with 1 and 2 doses of influenza subunit vac- cines with various hemagglutinin contents respectively ,0.5 ml for each, using those unimmunized as a control group. The interval be- tween doses was one week. Serum samples were collected 21 d after the first dose and determined for hemagglutination inhibition （HI） and neutralizing antibody titers. Results： The HI and neutralizing antibody titers of mice immunized with 1 and 2 doses of H1N1 influ- enza subunit vaccine （hemagglutinin contents of 20μg/ml） were less than 1：40 and 1：200 respectively. However,the HI antibody ti- ters of mice immunized with ! and 2 doses of H1N1 influenza subunit vaccine （hemagglutinin contents of 30, 40μg/ml） were more than 1 ： 40, and nuetralizing antibody titers were more than 1 ： 200. Neither HI nor neutralizing antibody titers showed significant differ- ence in the mice immunized with 1 and 2 doses of H1N1 influenza subunit vaccine （hemagglutinin contents of 30, 40μ g/ml）. Con- clusion： The immune effects of one dose of H1N1 influenza subunit vaccine with hemagglutinin contents of 30μg/ml are satisfactory.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2013年第6期615-617,共3页
Chinese Journal of Immunology
基金
长春市社会发展科技计划资助项目(10SF03)
关键词
流感病毒亚单位疫苗
血凝素
小鼠
免疫原性
H1N1 Influenza subunit vaccine
Hemagglutinin
Mice
Immunogenicity
