摘要
目的检测Calpain I、Calpain II、caspase-12在风湿性心脏病心房颤动患者心房组织中的表达,探讨其在心房纤维化发展中的作用及其对房颤发生、维持的作用。为临床慢性房颤患者心房纤维化及心房结构重构的防治提供新的靶点。方法取32例风湿性心脏病行外科换瓣手术患者左心房组织,分为A组:永久性房颤组(Permanent AF组)16例,B组:阵发性或持续性房颤组(Paroxysmal or persistent AF组)10例,C组:窦性心律组(Sinus rhythm组)6例。采用免疫组化法测Calpain I、Calpain II、caspase-12在左心房组织中的表达并计算其灰度值,心肌胶原染色采用VG染色,光镜下观察组织纤维化程度,并计算胶原容积积分(CVF)。结果风湿性心脏病心房颤动患者calpain I、calpain II、caspase-12在左心房组织表达明显增多(P<0.05),calpain I、calpain II与CVF呈正相关。结论 calpain I、calpain II通过促进心肌细胞凋亡促进了风湿性心脏病心房颤动心房纤维化过程。
Objective To detect the expression of calpain I,calpain II,caspase-12in atrial tissues of Patients with Atrial Fibrillation during Rheumatic Heart Disease,for the purpose of approaching the role of which in the development of atrial fibrosis and its effect on the development of atrial fibrillation,through the way,we could get a new therapeutic target to prevent atrial fibrosis and atrial structural remodeling of patients with chronic atrial fibrillation.Methods The left atrial tissue samples were taken from 32 patients with rheumatic heart disease who underwent heart valve replacement surgery.which is divided in A group ：permanent atrial fibrillation group 16 cases,B group ：paroxysmal or persistent atrial fibrillation group 10 cases,C group：sinus rhythm group（Sinus rhythm;SR Group） 6 cases.The expression of Calpain I,Calpain II,caspase-12 in the left atrial tissue measured by immunohistochemical method and to calculate the gray value,VG staining were used for quantitative analysis of collagen accumulation and assessed the extent of atrial fibrosis.Results Expression of calpain I,calpain II and caspase-12 in the left atrial tissue of Patients with rheumatic heart disease（RHD） complicating chronic atrial fibrillation was significantly increased,calpain I,calpain II and CVF was a positive correlation.Conclusion calpain I,calpain II participate in the process of fibrosis in RHD complicating chronic atrial fibrillation through the promotion of cardiomyocyte apoptosis.
出处
《中国实验诊断学》
2013年第6期1039-1042,共4页
Chinese Journal of Laboratory Diagnosis
关键词
心房颤动
卡配因
Atrial fibrillation
calpain
