摘要
应用光化学原理,以自制冷光源(LG150B)诱导大鼠大脑中动脉血栓形成,并观察其后动物行为学改变、脑水肿程度、脑梗塞面积以及尿激酶和水蛭提取物的治疗作用。结果,光照诱导后4、12、24h均出现行为学改变,以24h时为著;脑水肿也以24h时最为严重,梗塞灶约占脑(嗅球、小脑和低位脑干除外)切片面积的10.51%±2.37%;尿激酶和水蛭提取物处理后以上改变均有明显改善。以水蛭提取物(2g/kg)处理可使脑水肿程度下降29%,梗塞面积缩小45%。结果表明用LG150B冷光源可成功建立大鼠大脑中动脉血栓模型,水蛭提取物可减轻其脑梗塞程度。
Cerebral thrombus model was duplicated by applying cold light to middle cerebral artery to induce thrombus in rats, and the effects of urokinase(UK) and an extract of Whitmania pigra Whitman were observed. The results showed that severe edema, infarction in cerebrum and neurobehavior disorders developed 24 hours after thrombus induction, and UK and the extract of Whitmania pigra could mollify these injuries. The extract of Whitmania pigra could decrease the extent of cerebrum edema by 29% and the infarction area by 45% (5.78%±0.88% vs 10.51%±2.37%,P<0.01) as compared with untreated model group. It suggested that the extract of Whitmania pigra might be used to treat the injuries from cerebral thrombus, and the Photochemically induced rat model of cerebral thrombus was successful.
出处
《上海实验动物科学》
1999年第4期197-200,共4页
Shanghai Laboratory Animal Science
