摘要
为了从早期胚胎寻找与发育分化有关的新基因,本文构建了3周龄人胚cDNA文库,并应用EST技术对该文库中随机挑选的47个低丰度克隆进行测序,结果发现了一个与人亚端粒DNA和锌指基因同源的cDNA克隆(L30),该基因长约3.8kb,5′端序列有明显的阅读框架(ORF),3′端序列有加尾信号(AAUAAA)和有39个A组成的Poly(A)尾巴;通过Northern杂交确认在早期人胚胎中有表达,应用地高辛染色体原位杂交技术将其定位于人第12号染色体长臂端部。
In order to isolate novel genes related to early human embryo development and differentiation, a directional cDNA library was constructed from 3-week-old human embryo. Single-pass DNA sequence analysis was used to sequence 47 randomly picked low-abundance cDNA clones. This approach led us to select a clone, L30, showing significant homology with the telomeric-
associated DNA and zinc finger protein genes. It is about 3. 8 kb in length and contains an open reading frame of notable length within 5' -region, and a tailing signal of AAUAAA and poly (A-) with 39 A in 3' -region. The gene was transcribed in human embryo by Northern blot hybridization and assigned to human chromosome 12 by in situ hybridization.
出处
《实验生物学报》
CSCD
2000年第1期27-34,共8页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金(39900082)
武汉大学自强基金~~
关键词
人早期胚胎
端粒DNA探针
锌指蛋白
EST
基因克隆
Early human embryo.Telomeric probes.Zinc finger gene.EST (expressed sequence tags).In situ hybridization.
