摘要
目的探讨壳聚糖对镉致小鼠睾丸损伤的干预作用。方法50只健康昆明种雄性小鼠,随机分为5组,即对照组,单纯镉染毒组,壳聚糖50、150、450mg/kg 3个剂量干预组。壳聚糖干预组分别灌胃50、150、450mg/kg壳聚糖,对照组和单纯镉染毒组灌胃蒸馏水。2h后单纯镉染毒组和各剂量壳聚糖干预组腹腔注射氯化镉0.8mg/kg,对照组腹腔注射蒸馏水。连续14d。末次染毒后处死动物,剖取附睾和睾丸,分别测定精子畸形率、睾丸超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量。结果小鼠染毒14d后,各处理组小鼠精子畸形率明显升高,与对照组比较,差异有统计学意义(P<0.05);睾丸SOD活力明显下降,其中单纯镉染毒组与对照组比较,差异有统计学意义(P<0.05);其余各剂量壳聚糖干预组与对照组比较,差异均无统计学意义(P>0.05)。MDA含量明显升高,与对照组比较,单纯镉染毒组、50和150mg/kg壳聚糖干预组差异有统计学意义(P<0.05)。随着壳聚糖摄入剂量的逐渐升高,小鼠精子畸形率有所降低,睾丸SOD活力有所升高,MDA含量有所下降,与单纯镉染毒组比较,差异均有统计学意义(P<0.05)。结论壳聚糖对镉致小鼠睾丸损伤有一定的干预作用。
Objective To explore the intervention of chitosan on cadmium induced testicle injury in mice. Methods 50 Kunming male mice were randomly divided into 5 groups including the control group, cadmium alone exposed group and 3 different dosages of chitosan intervention groups （50,150 and 450 mg/kg of chitosan）. 50, 150 and 450 mg/kg of chitosan were instilled into intervention groups respectively. The control group and cadmium alone exposed group were instilled with water. Two hours later the cadmium alone exposed group and three chitosan intervention groups were intraperitoneally injected with CdClz solution of 0.8 mg/kg. The control group was given intraperitoneally injection with distilled water. All the groups were treated for 14 days. At the 15th day mice were sacrificed. The epididymis and testis were separated. The rates of sperm abnormalities, the activities of superoxide dismutase （SOD）, and the contents of malondialdehyde （MDA） in testis were determined. Results After 14 days treatment, the rates of sperm abnormalities of every treated groups increased obviously compared with the control group, the difference was statistically significant （P〈0.05）. SOD activities in testicle decreased obviously. Among all the groups, the difference between the cadmium group alone exposed group and the control group was significant （P〈0.05）. In other chitosan intervention groups of different dosages, there was no statistical difference in comparison with the control group （P〉0.05）. As to the MDA contents in testicle, those of the cadmium alone exposed group and 50 and 150mg/kg of chitosan intervention groups were seriously higher than those of the control group （P〈0.05）. However, as the dosages of chitosan intake gradually increased, the rates of sperm abnormalities decreased, the testicular SOD activities increased and the MDA contents somewhat decreased in different dosages of chitosan intervention groups. Compared with the cadmium alone exposed group, the difference was statistically sig
出处
《工业卫生与职业病》
CAS
CSCD
北大核心
2013年第4期196-199,共4页
Industrial Health and Occupational Diseases
