摘要
西瓜子叶外植体能被含双元载体 p BPMWMV的根瘤农杆菌感染 .该质粒载体含有一个 npt- 基因以供选择 Kanr的转基因西瓜植株以及一个 WMV- 2 CP基因 . Kanr西瓜植株经 NPT- 酶活性检测、DNA分子点杂交及 Southern杂交试验证明 ,外源的 WMV- 2 CP基因已导入西瓜细胞 .
A simple and effective system for transformation and regeneration of Citrullus vulgaris Schrad has been developed. Segments of cotyledonary explants can be infected by agrobacterium tumefacienss harboring a binary vector containing a npt Ⅱ gene and a WMV 2 CP gene. Transgenic plants were obtained. Successful genetic transformation was confirmed by NPT Ⅱ enzyme activity assay, DNA dot hybridization and Southern blot analysis.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2000年第1期111-113,共3页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
浙江省自然科学基金资助项目! (3 93 10 5 )
关键词
西瓜
根瘤农杆菌
WWV-2
转基因植株
抗病育种
Citrullus vulgaris Schrad
agrobacterium tumefaciens
WMV 2
CP gene
transgenic plant
