摘要
采用微波辅助法从蓝莓中提取多糖,比较传统的双氧水法脱色和三氯乙酸-正丁醇法脱蛋白与聚酰胺柱层析法脱色脱蛋白的效果,蓝莓粗多糖经DEAE-52纤维素离子交换柱和Sephadex G-100凝胶柱分离纯化,应用高效液相色谱法鉴定纯度,气相色谱法分析单糖组成。结果表明:通过响应面优化设计确定最佳工艺条件为料液比1:8(g/mL)、微波功率600W、微波提取时间30min,此条件下提取量达到3.32%,聚酰胺柱层析法脱色脱蛋白效果明显优于传统方法。蓝莓多糖经DEAE-52纤维素离子交换柱分离得到3个级分BBP0、BBP1、BBP2,BBP0经Sephadex G-100凝胶柱进一步纯化得到蓝莓多糖BBP0-2组分,主要由阿拉伯糖、半乳糖、木糖和葡萄糖4种单糖组成,其物质的量比为2:5:3:4。
The microwave-assisted extraction of polysaccharide from blueberry fruit was optimized by respons surface methdology in this work. Hydrogen peroxide, trichloroacetic acid-n-butanol and polyamide column chromatography were compared for their efficiencies in removing pigment and protein from crude polysaccharide from blueberry fruit. After further purification by DEAE-cellulose 52 column and Sephadex G-100 column chromagraphy, we analyzed the purity by HPLC and monosaccharide composition of purifed polysaccharide by GC. The optimal conditions for polysaccharide extraction were found to be 1：8 （g/mL）, 600 W and 30 rain for solid/solvent ratio, microwave power and extraction time, respectively. The extraction efficiency of ploysaccharide under the optimized conditions was 3.32%. Polyamide column chromatography was considerably more effective than the traditional methods with hydrogen peroxide and trichloroacetic acid-n-butanol. Three polysaccharides were separated from DEAE-cellulose 52 column chromatography, namely BBP0, BBP1 and BBP2. BBP0 were further separated by Sephadex G-100 column chromagraphy to obtain BBP0-2, which was composed of arabinose, galactose, xylose and glucose with a molar ratio of 2：5：3：4.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第12期119-124,共6页
Food Science
基金
"十二五"国家科技支撑计划项目(2012BAD31B05)
辽宁省科技厅农业攻关项目(2011205001)
关键词
蓝莓
多糖
微波提取
分离纯化
blueberry
polysaccharide
microwave-assisted extraction
isolation and purification
