摘要
目的观察小鼠紫外光敏感视锥系统（UV-cone）视网膜电图（ERG）的特点。方法实验研究。成年野生型C57BL／6J小鼠10只（10眼），随机分成实验组和对照组，每组5只。2组均明适应（背景白光的色温为7000K，亮度为30cd·m-2）10min后行ERG检测。实验组记录UV-cone ERG，刺激所用的紫外光波长为363 nm，刺激强度分别为0．03、O．30、1．00、3．00mW·s·m-2。对照组记录明视系列白光ERG。2组间最大反应a波、b波振幅的比较采用独立样本t检验。结果实验组紫外光（UV）刺激强度达到0．30mW·S·m-2时，ERG开始出现稳定的正向b波，其振幅为（14．8±3．0）uV；随着刺激强度增大，b波上可以记录到明显的振荡电位（前3个子波振幅较大）。而对照组b波上未见明显的振荡电位。实验组ERG最大反应b波振幅明显高于对照组（t=2．615，P〈0．05），而a波振幅2组问差异无统计学意义（t=-1．633，P〉0．05）。结论正常成年C57BL/6J小鼠明视UV—coneERG与传统的白光ERG在波形和最大反应幅值上存在一定的差异。
Objective To observe the characteristics of photopic ultraviolet-sensitive cone electroretinograms （UV-cone ERGs） in C57BU6J mice. Methods This was an experimental study. Ten adult wild-type C57BL/6J mice （10 eyes） were divided into 2 groups of 5 each. Photopic ERGs were elicited after 10 minutes of adaptation under a steady white background illumination of 30 cd＇m-2. Mice in the experimental group displayed UV-cone ERGs elicited by UV flashes （K=363 nm） with stimulus intensities of 0.03 mW＇s.m-2, 0.30 mW＇s.m-2, 1.00 mW.s.m-2 and 3.00 mW＇s.m-2. Mice in the control group displayed white flash ERGs （stimulus intensities ranged from 0.02 to 10.00 cd.s.m-2）. The maximum responses of the a- and b-wave amplitudes of the 2 groups were compared. An independent samples t test was used for statistical analysis. Results As UV intensity increased to 0.30 mW＇s＇m-2, the UV-cone ERG could be elicited with an amplitude of 14.8±3.0 I^V. At an intensity of 1.00 mW＇s.m-2 or 3.00 mW.s.m-2, oscillatory potentials （OPs） with a sizeable Oi-3 were recorded on the b-wave. But there were no obvious OPs in the control group. The maximum response showed that the b-wave amplitude of the experimental group was significantly higher than that of the control group （t=2.615, P〈0.05）, although there was no statistically significant difference in a-waves （t=-1.633, P〉0.05）. Conclusion In adult C57BU6J mice, the difference between photopic UV and white flash ERGs involve wave shape and maximum amplitude. Our results provide normal reference values for UV-cone ERGs in mice.
出处
《中华眼视光学与视觉科学杂志》
CAS
2013年第6期336-338,共3页
Chinese Journal Of Optometry Ophthalmology And Visual Science
