摘要
目的 探讨脑源性神经营养因子（BDNF）基因G196A多态性与精神分裂症精神病理症状和认知功能的相关性.方法 采用聚合酶链反应技术（PCR）、非变性聚丙烯酰胺凝胶电泳结合银染技术,检测224例精神分裂症患者（研究组）和220名正常人（对照组）的BDNF基因G196A基因型和等位基因分布频率,采用阳性与阴性症状量表评定精神病理症状、威斯康星卡片分类测验（WCST）和连线测验（CPT）评定认知功能.结果 ①研究组和对照组BDNF基因G196A基因型G/G、G/A、A/A和等位基因G和A分布差异无统计学意义（均P＞0.05）.②阳性症状和阴性症状为主的精神分裂症患者之间BDNF基因G196AA/A基因型分布存在显著差异（x2=4.558,P＜0.05）.③G/G、G/A、A/A三种不同基因型患者的威斯康星卡片分类测验的完成分类数、持续错误数和两种连线测验成绩比较,差异均无统计学意义（均P＞0.05）.结论 BDNF基因G196A多态性与精神分裂症及其认知功能不相关,但与阳性症状相关.
Objective To explore the relationships between brain-derived neurotrophic factor gene G196A polymorphisms and psychopathology and the cognitive function in schizophrenic patients.Methods 224 patients and 220 normal controls were examined with polymerase chain reaction（PCR）,denaturing polyacrylamide gel electrophoresis and silver staining to determine genotype and allele of G196A.Clinical symptoms were assessed with the Positive and Negative Symptom Scale （PANSS）.Cognitive function was assessed with Wisconsin card sorting test （WCST） and Trail Making Test（CPT）.To analyse the differences of the scores of Scale for the Assessment of Positive Symptoms（SAPS） and Scale for the Assessment of Negative Symptoms （SANS） among the patients with the genotype G/G,G/A and A/A.At the same time,the differences of the performance of WCST and CPT were analyzed.Results ①There were no significant differences of the genotypes G/G,G/A and A/A and allele G and A between patients and controls.②There were significant differences of the genotypes A/A between patients with Positive Symptoms and patients with Negative Symptoms （x2 =4.558,P 〈 0.05）.③There were no significant differences in the performances of complete categorizations and persistent wrong numbers of WCST and the performances of CPT among three groups of patients with genotype G/G,G/A and A/A （One-Way ANOVA,all P 〉 0.05）.Conclusion BDNF gene G196A polymorphisms are not associated with schizophrenia and the cognitive function but are associated with positive symptoms of schizophrenia.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2013年第6期494-496,共3页
Chinese Journal of Behavioral Medicine and Brain Science
基金
基金项目：山东省医药卫生科技发展计划项目（2007HW037）
山东省自然科学基金（ZR2012HM065）
卫生部公益性行业科研专项经费项目（201002003）
关键词
精神分裂症
脑源性神经营养因子
认知功能
基因多态性
Schizophrenia
Brain-derived neurotrophic factor
Cognitive function
Gene polymor-phism
