摘要
采用实验生态学方法,开展了汞胁迫下拟穴青蟹(Scylla paramamosain)的消化和免疫因子变动的研究。在实验室条件下,测定了0.00、0.005、0.01、0.02、0.04、0.08mg/L浓度Hg2+在1d、3d、5d、7d、9d胁迫时间下的消化和免疫因子变化。结果表明,汞胁迫下拟穴青蟹AMS活性快速升高,但激活效应随胁迫时间延长而逐渐下降,后期表现为抑制效应。汞胁迫能激发Pepsin活性,短期内Pepsin活性和胁迫浓度、胁迫时间呈正相关;长期高浓度胁迫(0.04、0.08mg/L),则激发效应减弱。汞胁迫对LPS活性的影响主要表现为抑制作用。免疫因子的研究结果表明,汞胁迫对AKP有激发作用,可迅速刺激机体AKP活力上升。低浓度汞胁迫对AKP活性提升最显著(0.005、0.01、0.02mg/L),高浓度组AKP活性先升后降。汞胁迫对ACP活性有激发作用,且存在显著的时间效应和浓度效应。汞胁迫下SOD活性短期内即显著升高,且SOD活力随胁迫时间延长而持续升高。汞胁迫对LZM活性有激活作用,且激活效应存在时间效应和浓度效应。汞胁迫对PO活力快速产生抑制作用,抑制作用存在时间效应而无显著的浓度效应。汞胁迫对拟穴青蟹消化和免疫因子能产生胁迫效应,对Pepsin、AMS、AKP、ACP、SOD、LZM表现为激发,而对LPS、PO表现为抑制。
An experimental ecology method was conducted to investigate the main digest factors and immune factors activities of Scylla paramamosain exposed to different concentrations of water-borne Hg^2＋ （0.005, 0.01, 0.02, 0.04, 0.08mg/L） from ld to 9d with the natural seawater （salinity 16±1, pH 7.5±0.3） as control. The results showed that the ac- tivities of AMS in stomach increased rapidly but the activation effect declined with the extending of the experiment, and inhibitory effect was observed at the late period of the experiment （9d）. The Pepsin activities in stomach were increased and positive correlated with the experimental period and Hg^2＋ stress intensity, but the Pepsin activities in high Hg^2＋ stress intensity （0.04, 0.08mg/L） groups declined near to the control group at the end of the experiment. The LPS activities in the stomach were declined by Hg^2＋ stress and the effective enhanced with the experimental period. The results of immune fac-tors analysis showed that the activities of AKP in hepatopancreas were increased rapidly, and lower Hg^2＋ stress intensity groups （0.005, 0.01, 0.02mg/L） had the most significant improvement of AKP activities, the AKP activities in higher Hg^2＋ stress intensity groups （0.04, 0.08mg/L） increased at first and declined at the end. The activities of ACP in hepatopancreas were increased by Hg^2＋ stress, and the time effect and dosage effect is conspicuous. The activities of SOD increased im- mediately （ld） in Hg^2＋ stress and increased gradually during the whole experiment. The activities of LZM were increased and the time effect and dosage effect was visible. Nevertheless, the activities of PO declined by Hg^2＋ stress, and gradually reduced during the experiment, but there was no significant correlation between the PO activities and the Hg^2＋ stress in- tensity. In summary, Hg^2＋ stress affected the activities of digest factors and immune factors of S. paramamosain, the activi-ties of Pepsin, AMS, AKP, ACP, SOD and LZM were
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2013年第3期747-754,共8页
Oceanologia Et Limnologia Sinica
基金
福建省科技厅青年创新项目
2008F3077号
福建省教育厅科技项目
JA09158号
国家自然科学基金项目
40376044号
关键词
汞
拟穴青蟹
消化因子
免疫因子
Hg^2＋
Scylla paramamosain
digest factors
immune factors
