摘要
目的 探讨 mdm2与 p5 3基因在骨骼肌恶性肿瘤中的改变及其临床意义 .方法 采用 RNA打点杂交技术分析2 0例骨骼肌恶性肿瘤中的 mdm2与 p5 3基因的表达水平 ,采用 PCR- SSCP技术分析 p5 3基因的突变情况 ,结合随访结果 ,分析其临床意义 .结果 1RNA打点杂交结果 :mdm2基因在 10例恶性肿瘤标本中呈高表达 ,在 8例肿瘤标本与 2 0例瘤旁组织标本中呈低表达 ,mdm2的表达在骨骼肌恶性肿瘤中显著高于瘤旁组织 (P<0 .0 1) ;p5 3基因在 12例骨骼肌肿瘤标本中呈高表达 ,在相应瘤旁组织中呈低表达 ,两者之间存在显著性差异 (P<0 .0 1) ;2 PCR- SSCP未检出 mdm 2基因突变 ,检出 9例肿瘤标本中存在 p5 3基因突变 ;33a随访结果 :9例p5 3突变患者 ,3例死于术后 0 .5 a,4例死于术后 1a,2例死于术后 1.5 a,11例无 p5 3突变患者 ,6例死于术后 2 a,1例死于术后 3a,4例 3a后尚存活 .结论 mdm2基因以高表达方式 ,p5 3以突变、高表达方式参与骨骼肌恶性肿瘤发生发展 ,检测 mdm2有无高表达与 p5 3有无异常可辅助判断运动系统肿瘤患者的预后 .
AIM To explore the alteration of mdm 2 and p53 in musculoskeletal tumors and its clinical significance. METHODS By using RNA dotblot and PCR SSCP, we analyzed the alteration of mdm 2 and p53 in 20 specimens of musculoskeletal tumors and paired peritumorous tissues and made a follow up study of the patients for 3 years after the operation for the clinical significance of the change. RESULTS By using RNA dotblot, it was found that mdm 2 was overexpressed in 10 specimens of musculoskeletal tumors, lowexpressed in 8 specimens of musculoskeletal tumors and 18 of peritumorous tissues, indicating that there was a significant difference between the mdm 2 expressions in bone tumors and peritumorous tissues ( P <0.01). p53 was overexpressed in 12 specimens of musculoskeletal tumors and lowexpressed in 20 specimens of peritumorous tissues, indicating that there was a significant difference between p53 expressions in bone tumors and peritumorous tissues ( P <0 01). PCR SSCP analysis didn't show any mdm 2 mutation in the specimens, but in 9 specimens of bone tumors p53 mutation was discovered. Results of the 3 year follow up study: 3 of the 9 patients with mutation of p53 died 6 months after the operation and 4 died 12 months after the operation and 21.5 years after the operation. 6 of the 11 patients without mutation of p53 died at 2 years after the operation and 1 died 3 years later, and 4 are still alive. CONCLUSION mdm 2 gene takes part in the progress of musculoskeletal tumors by overexpression and p53 gene by mutation and overexpression. It is probably helpful for prognosis to screen mdm 2 overexpression and p53 alteration in musculoskeletal tumors.
出处
《第四军医大学学报》
2000年第8期1002-1004,共3页
Journal of the Fourth Military Medical University
