摘要
[Objective] This study aimed to investigate the influence of illumination intensity, 5-aminolevulinic acid （ALA） concentration and their interaction on chlorophyll fluorescence parameters and yield of summer maize. [Method] Two illumination intensity levels and five ALA concentrations were applied in the experiment using 2x5 completely balanced program. The two illumination intensity levels were natural light （So） and 60% shade （SO, and five ALA concentrations were 0, 1, 10, 25 and 50 mg/L. [Result] The relative chlorophyll content of leaf （SPAD）, the optimal/maximal quantum yield of PS II （Fv/Fm）, the photochemical quenching coefficient （qP）, electron transport rate （ETR）, grain number per cob and grain weight per cob in $1 treatment were significantly reduced compared with that in So. However, the non- photochemical quenching coefficient （qN） was significantly increased. The responses of these parameters to ALA were different under So and $1 treatments. The SPAD, Fv/Fm, qP, ETR, grain number and grain weight per cob were firstly increased, but then decreased following the raise of ALA concentration, ranging from 0 to 50 mg/L, whereas qN showed opposite trend. The effect of the interaction of illumination in- tensity and ALA concentration on these parameters was significant （P〈0.05）. Under natural light, summer maize could obtain higher SPAD, Fv/Fm, qP and ETR and lower qN combined with low concentration of ALA. However, high concentration of ALA was needed under shading to get the same results. [Conclusion] Soaking seed in suitable concentration of ALA can reduce the yield loss of summer maize caused by short-term shading in seedling stage.
[目的]研究光、5-氨基乙酰丙酸（ALA）及其交互作用对夏玉米叶绿素荧光及产量的影响。[方法]试验采用照光强度和ALA浸种浓度2×5完全均衡方案,光强度设自然光（S0）和遮光60%（S1）2个水平,ALA浸种浓度设0、1、10、25、50mg/L5个水平,研究光照强度、ALA浸种浓度及其交互作用对夏玉米苗期叶绿素SPAD值、叶绿素荧光参数及产量的影响。[结果]与S0相比,S1处理显著降低了夏玉米叶绿素SPAD值、叶绿素荧光参数Fv/Fm、qP、ETR、每穗籽粒数和籽粒重,显著提高了叶绿素荧光参数qN;在2种光照条件下,上述指标对ALA浸种浓度的响应特点不同,在ALA浸种浓度0~50mg/L范围内,叶绿素SPAD值、叶绿素荧光参数Fv/Fm、qP、ETR、每穗籽粒数和籽粒重均随着浸种浓度的升高呈现先升高后降低的趋势,而叶绿素荧光参数qN表现规律则与前者相反;光强度和ALA浸种浓度对上述指标影响的交互效应均达显著水平,在自然光条件下,夏玉米达到较高的叶绿素含量、叶绿素荧光参数Fv/Fm、qP、ETR以及较低的叶绿素荧光参数qN仅需以较低浓度的ALA浸种,而遮光条件下则需要以较高浓度的ALA浸种。[结论]遮光条件下采用适宜浓度的ALA浸种可弥补苗期短期遮光造成的夏玉米产量损失。
基金
Supported by National Key Technology Research and Development Program(2011BAD10B07)~~
