摘要
为选育抗寒能力较强的栽培品种,以香玲、西林3号、鲁W06-1、晋龙、清香、西洛3号、青7-2#、青-16#及青林等9个核桃栽培品种优系一年生休眠枝条为试验材料,分别测定了材料中组织含水量,以及经-10℃、-15℃、-20℃、-25℃、-30℃处理后材料相对电导率(REC)、丙二醛含量(MDA)、超氧化物歧化酶活性(SOD)、游离脯氨酸和可溶性糖含量。结果表明:随处理温度降低,枝条相对电导率和丙二醛含量增加,超氧化物歧化酶活性、游离脯氨酸和可溶性糖含量先增加后下降。9个品种优系的自由水与束缚水比值范围为0.83～1.61,半致死温度(LT50)为-17.196℃～-24.484℃,品种优系间差异显著。在-20℃处理下,鲁W06-1和晋龙的SOD活性快速增大至对照的2.5倍以上,游离脯氨酸浓度大于90μg.g-1,分别为对照的2.31倍和3.05倍,抗寒性较强。根据隶属函数综合评价,9个品种优系的抗寒性强弱为:鲁W06-1>晋龙>清香>青-16#>西林3号>青林>青7-2#>香玲>西洛3号。
In order to select high cold-tolerance walnut cultivars in Shaanxi Province, nine walnut cultivars and superior lines were studied, including Xiangling, Xilin NO.3, Lu W06-1, Jinlong, Qingxiang, Xiluo NO.3, Qing7-2#, Qing -16# and Qinglin. Annual branches of nine walnut cultivars and superior lines were treated respectively at -10℃、-15℃、-20℃、-25℃ and -30℃. The results show that the relative electric conductivity（REC） and malondialdehyde content（MDA） increased when the temperature became lower, while superoxide dismutase（SOD） activity, free proline and soluble sugar contents firstly increased then decreased; Among cultivars and superior lines there were sigalificant differences in the ratio of free water content to bound water content and semi-lethal temperature （LTs0）, the ratio of free water content to bound water content was intervenient 0.83 and 1.61, and the range of LTs0 was -17,20 to -24.48 ℃ ; Under -20 ℃ , SOD activity of Lu W06-1 and Jinlong increased rapidly to its maximum value that was over 2.5 times of the control group, and their free proline content was more than 90 μg.g1, which was 2.31 times and 3.05 times of the control group respectively. According to a comprehensive evaluation based on subordinate function value analysis, their cold resistance from high to low was： Lu W06-1, Jinlong, Qingxiang, Qing-16#, Xilin NO.3, Qinglin, Qing 7-2#, xiangling and Xiluo NO. 3.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2013年第4期50-55,共6页
Journal of Central South University of Forestry & Technology
基金
国家林业公益性行业科研专项(201004027)
关键词
核桃
抗寒性
生理特性
隶属函数法
Juglans regia L.
cold resistance
physiological property
subordinate function
