摘要
目的:研究巴曲酶(batroxobin)对兔VX2肝癌生长的抑制作用。方法:采用CT引导下种植移植瘤的方法建立兔VX2肝癌移植瘤模型;建模3周后,经CT评估将种植成功的兔子随机分为2组,即药物组(经兔耳缘静脉注射巴曲酶0.1BU/kg)和对照组(经兔耳缘静脉注射等体积0.9%氯化钠溶液),观察巴曲酶对VX2肝癌移植瘤兔中纤维蛋白原(brinogen,FIB)含量的影响;计算巴曲酶干预前1d及干预后28d时肿瘤的体积及增长率,并对比肿瘤病灶体积的变化情况;巴曲酶干预后第28d后处死荷瘤兔,计数肺表面转移性结节个数。结果:VX2肝癌种植前1d及巴曲酶干预前1d,2组实验兔中FIB的含量差异无统计学意义(P>0.05)。药物组在干预后第4天、第10天及第28天时,FIB水平与干预前1d相比,差异均有统计学意义(P<0.05),而对照组在同一时间点FIB的水平差异则无统计学意义(P>0.05)。干预前2组实验兔肿瘤的体积差异无统计学意义(P>0.05),干预后第28天,2组肝癌移植瘤的体积均有增大,差异有统计学意义(P<0.05),但药物组的肿瘤体积增长率小于对照组,两者比较差异有统计学意义(P<0.05)。2组移植瘤兔中均见肺部转移结节,肺部转移率均为100%。其中,药物组肺表面转移结节数为(73.8±8.8)个,对照组肺表面转移结节数为(87.8±9.6)个,对照组肺部转移性结节数明显多于药物组,2组比较差异有统计学意义(P<0.05)。结论:去纤酶可以抑制兔VX2肝癌的生长及转移。
To investigate the inhibitory effect of batroxobin on the growth of VX2 liver cancer inrabbits. Methods： The model of rabbit bearing VX2 liver cancer was established by CT-guided transplant. Three weeks later, the rabbits bearing VX2 liver cancer successfully were randomized into study group （injection of batroxobin at a dose of 0.1 BU/kg via marginal ear veins of the rabbit） and the control group （injection of normal saline of the same volume via marginal ear veins of the rabbit）. The change of FIB （fibrinogen） content in liver cancer of the study group was observed. The volume and growth rate of the liver cancer one day before and 28 days after batroxobin treatment were obseved and calculated. On the 28th day after batroxobin treatment, the rabbits were sacrificed and the number of metastatic nodules on pulmonary surface was recorded. Results： The content of FIB was not different between the two groups one day before the transplant or batroxobin treatment （P 〉 0.05）. The contents of FIB in the study group on the 4th, 10th and 28th days after batroxobin treatment were significanly different from one day before batroxobin treatment （P 〈 0.05）, but this effect was not observed in the control group （P 〉 0.05）. Before batroxobin treatment, the volume of liver cancer in rabbit was not different between the two groups （P 〉 0.05）, but it was increased on the 28th day after batroxobin treatment in both groups（P 〈 0.05）, and the growth rate of the liver cancer in the study group was lower than that of the control group （P 〈 0.05）. The metastatic lung lesions were observed in both groups, the metastatic rate was 100%. The mean numbers of the metastatic nodules on pulmonary surface in the study group and the control group were 73.8___8.8 and 87.8_＋ 9.6, respectively （P 〈 0.05）. Conclusion： Defibrase may inhibit the growth and metastasis of VX2 liver cancer in rabbit model.
出处
《肿瘤》
CAS
CSCD
北大核心
2013年第6期515-519,共5页
Tumor
