摘要
应用双生病毒外壳蛋白基因保守序列设计的简并引物对广东省广州、中山、珠海、东莞、深圳等5个地区20个采样地点的100个扶桑黄化曲叶病样本进行PCR检测,将获得的产物克隆到pGM-T载体中,对其进行测序和序列分析。结果表明:广东地区侵染扶桑导致黄化曲叶症状的病毒是木尔坦棉花曲叶病毒CLCuMV,来自5个地区的病毒核苷酸序列同源性均达97.5%以上,其中19个采样点78份样品检测出特异片段,样品的阳性检出率为78%,样品采集地阳性检出率为95%,表明木尔坦棉花曲叶病毒感染导致的扶桑黄化曲叶病发生较为普遍。
According to the conserved sequence of coat protein genes of geminivirus, a pair of degenerate primers was designed and used to detect the presence of CLCuMV. 100 Hibiscus rosa-sinensis yellow leaf curl disease samples were collected from 20 sampling points in Guangzhou, Zhongshan, Zhuhai, Dongguan and Shenzhen. Detection was done by PCR technology, and then PCR products were cloned into the vector pGM-T. Sequence analysis indicated that the nucleotide sequences of the amplified products from above five cities had 97.5% identity, and shared 99% sequence identity with CLCuMV, which indicating the virus infected the hibiscus was CLCuMV. The DNA specific sequence was presented in 78 samples from 19 sampling points, and the positive rates in all samples and regions were high with 78% and 95%. That meant Hibiscus rosa-sinensis yellow leaf curl disease was common in Guangdong.
出处
《广东农业科学》
CAS
CSCD
北大核心
2013年第10期80-82,共3页
Guangdong Agricultural Sciences
基金
广东省植物防疫检疫研究专项(粤农计[2011]90号)
关键词
扶桑
木尔坦棉花曲叶病毒
PCR
检测
Hibiscus rosa-sinensis
Cotton leaf curl Mu｜tan virus （CLCuMV）
PCR
detection
