摘要
目的:评价ELISA定量检测甲胎蛋白(AFP)在普查早期肝癌中的临床应用。方法:用ELISA(双抗体夹心酶联免疫吸附法)对9580例体检者进行AFP浓度检测,对ELISA检测阳性的样本用化学发光微粒子免疫分析法(CLEIA)重新检测AFP水平,并对结果进行统计对比分析。结果:ELISA法定量检测AFP在体检人群中有2.23%的阳性率,ELISA与CLEIA检测AFP阳性例数差异无统计学意义,AFP>300μg/L确诊肝癌发生率达100%。结论:ELISA定量检测AFP对早期肝癌诊断率高,分AFP浓度段诊断肝癌准确性高,AFP>300μg/L肝癌发生可能性达100%,试剂成本低,操作简便快速,适合大批量体检普查应用。
Objective： To evaluate the clinical application in early hepatocellular carcinoma （ HCC ）census by using ELISA quantitative detection for alpha-fetoprotein （AFP） .Method： To conduct AFP concentration detection for 9580 health examinees by using ELISA.Re-detect the AFP level for positive samples among 9580 examinees through chemiluminescent micro particle immunoassay （ CLEIA ） .Then the results were summarized, compared and analyzed.Result： Quantitative detection for alpha-fetoprotein （ AFP ） by using ELISA method poses 2.23% positive rate among health examinees. There was no apparent difference between ELISA and CLEIA to detect AFP positive rate. The incidence of hepatocellular carcinoma （ HCC ） was up to 100% for those health examinees whose AFP rate was more than 300 μg/L.Conclusion： The diagnostic accuracy of early hepatocellular carcinoma （ HCC ） is high by using ELISA quantitative detection for alpha-fetoprotein （ AFP ） . The possibility of hepatocellular carcinoma is up to 100% for those whose AFP is higher than 300 μg/L. The reagent cost is low, the operation is simple and rapid, and it is suitable for mass health examination＇ s census application.
出处
《中国医学创新》
CAS
2013年第17期90-91,共2页
Medical Innovation of China
关键词
ELISA
甲胎蛋白
化学发光法
体检普查
应用价值
ELISA
AFP （ alpha fetoprotein ）
Chemiluminescence
Health examination census
Application value
