摘要
目的:建立以氨基柱反相高效液相色谱法测定单唾液酸四己糖神经节苷脂钠注射液含量的方法。方法:色谱柱为phenomenex NH2100A(4.6mm×250mm,5μm),流动相为乙腈-0.1mol/L磷酸水(66:34),流速1.0mL.min-1,检测波长为205nm。结果:单唾液酸四己糖神经节苷脂钠在52.3至836.8ug.mL-1浓度范围内线性关系良好,回归方程为:Y=32819X+64247(r=0.9992);平均回收率为98.32%,RSD=1.49%(n=9)。结论:该方法准确、简便、快速、可靠,能有效控制单唾液酸四己糖神经节苷脂钠注射液的质量。
Objective： To establish an HPLC method for detecting manosialotetrahexosylganglioside sodium injection. Methods： A phenomenex NH2 100A column （ g. 6mm x 150mm, 5tan } was used, and the mobile phase was acetonitrile - water（66：34）, column temperature was room temperature, at a flow rate of 1. 0ml. win and detection wave length of 205 tam. Results The linear range of monosialotetrahexosylganglioside sodium was 52.3 - 836 8ug＇mL- 1 ;The regression equation was as follows： ： Y = 32819X ＋ 64247 （ r = 0. 9992 ）. The average recovery of monosialotetrabexosylganglioside sodium was 98.32 %, RSD = 1.49%（n = 9}. Conclusion： The method is accurate, simple, rapid and suitable for the quality control of monosialotetrahexosylganglioside sodium injection.
出处
《激光杂志》
CAS
CSCD
北大核心
2013年第3期90-91,共2页
Laser Journal
