摘要
目的探讨子宫内膜癌(EC)患者NF-κB传导通路相关基因多态性与EC发生发展的关系。方法选择EC患者100例(EC组)、健康体检者100例(对照组)为研究对象,采用PCR-RFLP技术检测两组NF-κB传导通路上的NF-κB(-94ins/delATTG)、TNF-α(G-308A)、一氧化氮合酶(iNOS,C608T)、环氧化酶-2(COX-2,T8473C)位点的基因多态性,并进行对比分析。结果两组NF-κB(-94ins/delATTG)位点基因多态性出现频率比较有统计学差异(P<0.05),TNF-α(G-308A)、iNOS(C608T)、COX-2(T8473C)位点基因多态性出现频率比较无统计学差异(P均>0.05)。结论 NF-κB传导通路NF-κB(-94ins/delATTG)位点的基因多态性与EC的发生发展有关。
Objective To investigate the relationship between genetic polymorphisms of NF-κB signaling pathway and endometrial carcinoma in patients. Methods A total of 100 patients with endometrial carcinoma （EC group） and 100 healthy female individuals （ control group） were voluntarily recruited. Genetic polymorphisms of NF-κB （ -94ins/delAT- TG）, TNF-α （G-308A）, （iNOS, C608T） and （COX-2, T8473C） in NF-κB signaling pathway were detected by PCR- RFLP technique and then comparative analysis was conducted. Results There were statistically significant differences in the frequency of occurrence of NF-KB （-94ins/delATl＇G） genetic polymorphism between the two groups （P 〈 0.05 ）. In addition, no statistically significant differences were observed in genotypes and allele frequencies at TNF-α （G-308A）, iN- 0S（C608T） and COX-2 （T8473C） （ all P 〉0.05）. Conclusion Genetic polymorphisms of NF-κB（-94ins/delATl＇G） in NF-κB signaling pathway are associated with endometrial carcinoma.
出处
《山东医药》
CAS
2013年第17期12-14,共3页
Shandong Medical Journal
基金
广西壮族自治区自然科学基金面上项目(2011GXNSFA018253)
