摘要
目的探讨腹腔镜胃癌根治术患者外周血CK19mRNA的变化及意义。方法采用荧光定量聚合酶链反应(FQ-PCR)技术检测CK19mRNA在腹腔镜组(14例)及开腹组(37例)胃癌根治术外周血的表达情况。结果两组间仅手术时间差异有统计学意义(P<0.01),术中失血量及清除淋巴结数的差异均无统计学意义(均P>0.05)。腹腔镜组患者手术前后CK19mRNA的表达无明显变化(P>0.05)。开腹组患者手术前后CK1 9mRNA的表达差异有统计学意义(P<0.05)。开腹组术后CK1 9mRNA阳性表达患者明显多于腹腔镜组,差异有统计学意义(P<0.05)。结论与开腹手术相比,腹腔镜胃癌根治术可以降低静脉血CK19mRNA的阳性率,减少术后肿瘤血循微转移的危险性。
Objective To investigate the expression of CK19 mRNA in patients with gastric cancer undergoing laparo-scopic radical resection and its significance. Methods The expression of peripheral blood CK19mRNA was detected by fluo- rescence quantitative polymerase chain reaction （FQ-PCR） technology in patients with gastric cancer undergoing laparoscopic radical resection（laparoscopy group, n=14） or laparotomy resection（laparotomy group, n=37）. Results The operation time in la- paroscopy group was shorter than that in laparotomy group （P〈0.01）, while there were no significant differences in e intraopera- tive blood loss and number of removed lymph node between two groups （P 〉0.05）. There was no difference in expression of CK19mRNA before and after operation in laparoscopy group （P 〉0.05）, while there was statistically significant in laparotomy group （P〈O.05）. The rate of positive CK19mRNA expression in laparotomy group after operation was higher than that in laparo- scopic group （P〈0.05）. Conclusion Compared to laparotomy, laparoscopic radical resection of gastric cancer can reduce the positive rate of CK19mRNA expression in peripheral blood and the risk of tumor micrometastasis after operation.
出处
《浙江医学》
CAS
2013年第9期762-764,共3页
Zhejiang Medical Journal
