摘要
通过体外转录得到籼稻品种２３２蜡质基因第一内含子５’端４３０ｂｐ的ｓｓＲＮＡ分子，以及在此区域发生了自然突变的粳稻品种寒丰蜡质基因第一内含子５’端同样长度的ｓｓＲＮＡ分子。部分变性胶电泳结果表明两种ｓｓＲＮＡ分子的迁移速率不同。将两种ｓｓＲＮＡ分子的核酸序列用计算机分析，表明此两种ｓｓＲＮＡ分子能形成不同的茎环结构，自由能值也有差异。对突变引起的这些不同与两种水稻品种蜡质基因转录本剪接的差异进行了讨论。
Two 430 nucleotides ssRNAs were produced in vitro from two 430 bp DNA fragments containing the 5' part of intron 1 of rice cultivars 232 and Hanfeng, respectively. Nucleotide sequences of the Wx intron 1 in cultivar 232 (high amylose content) and Hanfeng (intermediate amylose content) differ by sixteen individual bases. While these two ssRNA molecules were resolved by electrophoresis in partially denatured polyacrylamide gel, showing that they migrated differently (Fig.2). Computer analysis of the secondary structure of RNA showed that the 5' region of the Wx RNA sequence in intron 1 of cultivar 232 could form a stem loop structure, which differs substantially from that of cultivar Hanfeng. The free energy values of the most stable structures of these two ssRNAs are different, too (Fig.3). Differences in RNA structure and splicing patterns of Wx transcript caused by the sixteen individual bases mutations are discussed.
基金
国家自然科学基金!资助( 项目批准号39893320)
