摘要
从断奶大鼠的肝脏中纯化得到了肝刺激物质(HSS)的有效成份,即肝再生增强因子(ALR)的蛋白质,酶解并对其多肽末端测序,据此推导出简并核苷酸序列,合成探针,对大鼠肝脏来源的cDNA文库进行筛选,首先获得了大鼠ALR的cDNA克隆,随后又分别克隆了人和小鼠的ALR的cDNA。与此同时,从酵母细胞中克隆了与线粒体氧化———磷酸化功能密切相关的ERV1基因,然后克隆了人的ERV1同源基因,从功能上证实人ERV1基因可以替代酵母细胞ERV1的功能,拯救酵母细胞ERV1基因突变。序列分析表明人ERV1基因与ALR是同一种基因。最近又克隆了酵母的ERV2基因,虽然还没有克隆到人及动物的ERV2基因,研究资料提示可能有ERV1基因超家族的存在。
Hepatic stimulating factor (HSS),the functional component of HSS,has been purified from weanling rat liver tissue.Degenerate primers have been designed according to the peptide digestion and partial sequence analysis,and rat ALR cDNA has been cloned from a cDNA library.And human and mouse ALR cDNA have been cloned thereafter.Meanwhile scERV1 cDNA has been cloned from yeast and found ERV1 is a functional gene related to mitochondrial function and phosphorylation.Human homologous ERV1 or ALR,could rescue the yeast with mutated scERV1.Recently scERV2,a ERV1 related gene has been cloned from the yeast,indicating perhaps a ERV1 gene superfamily existed although human,rat and mouse ERV2 equivalent has noe been reported.
出处
《生物学杂志》
CAS
CSCD
2000年第1期4-6,共3页
Journal of Biology
