摘要
目的:探讨肌球蛋白轻链激酶(myosin light chain kinase,MLCK)水平与冠状动脉粥样硬化性心脏病(冠心病,CHD)间的相关性。方法:将72例行冠状动脉造影术的患者根据造影结果分为对照组及CHD组,CHD组患者根据病情再分为急性冠脉综合征(acute coronary syndrome,ACS)和稳定性CHD 2个亚组。采集对照组患者入院24 h内及造影后24 h内血样和CHD组患者入院24 h内、造影后24 h内及入院第5天血样,应用双抗体夹心ABC-ELISA法测定其血清MLCK水平。结果:血清MLCK水平与血脂、血糖水平相关,且对CHD有一定诊断价值;院内发生不良事件的CHD患者血清MLCK水平显著升高,且ACS患者血清MLCK水平高于稳定性CHD组,而后者高于对照组(P均<0.05)。同时,血清MLCK与高敏C反应蛋白(hsCRP)间呈显著正相关。结论:血清MLCK水平可能与炎症反应及动脉斑块的不稳定性相关,对CHD的诊断和院内预后评估有一定价值。
Objective To study the relationship between myosin light chain kinase （MLCK） and coronary atherosclerotic heart disease （CHD）. Methods A total of 72 patients undergoing coronary angiography （CAG） were enrolled and divided into two groups, control group and CHD group,the CHD group was subdivided into ACS （acute coronary syndrome）group and stable CHD group.In control group, blood samples were collected within 24 hours after admission and at the CAG.In CHD group, blood samples were collected within 24 hours after admission,at the CAG and on the 5th day of hospitalization. Serum MLCK level was determined by ABC-ELISA double antibody sandwich method. Results Serum MLCK level was correlated with blood lipid and glucose level, and was valuable for the diagnosis of CHD; serum MLCK level was significantly higher in CHD patients with adverse events than those without adverse events.Serum MLCK level was higher in ACS group than in stable CHD group, and that in stable CHD group was higher than that in control group.Also, serum MLCK level was correlated with serum hsCRP level. Conclusions MLCK is likely to be related to inflammatory response and is involved in the instability of atherosclerotic plaque. Therefore, serum MLCK level may be valuable for the diagnosis and in-hospital prognosis prediction in patients with CHD.
出处
《诊断学理论与实践》
2013年第1期52-56,共5页
Journal of Diagnostics Concepts & Practice
基金
上海市科委"创新行动计划"(09140901700
09140902900)
国家自然科学基金(青年基金)(30900491)
上海市科委基础重点研究项目(10JC1410502)
