摘要
为了解恩诺沙星对异育银鲫(Carassius auratus gibelio)的急性毒性及血液生化指标的影响,评价恩诺沙星对异育银鲫的安全性。本实验研究了恩诺沙星对异育银鲫的急性毒性,并观察了恩诺沙星在不同剂量下对异育银鲫血液生化指标的影响。实验结果表明,恩诺沙星对异育银鲫的半数致死剂量为1 949.84 mg/kg,安全剂量为194.98 mg/kg。当恩诺沙星以常规给药剂量(20 mg/kg)连续口灌异育银鲫30 d时,异育银鲫的血清总蛋白含量、谷丙转氨酶含量、谷草转氨酶、γ-谷氨酰转移酶含量与对照组没有显著变化;而当恩诺沙星以40 mg/kg、80 mg/kg、160 mg/kg、320 mg/kg的剂量连续口灌异育银鲫30 d时,异育银鲫的血清谷丙转氨酶含量较对照组分别增加了23%、30%、46%、86%(P<0.05),血清谷草转氨酶含量较对照组分别增加了37%、42%、69%、86%(P<0.05)。本研究确定了恩诺沙星对异育银鲫的安全剂量,证实了恩诺沙星引起异育银鲫出现肝功能失调的剂量范围,对恩诺沙星在异育银鲫养殖中的安全使用提供了科学依据,也证实常规给药剂量(20 mg/kg)的恩诺沙星不会导致异育银鲫出现肝损伤。
To evalauate the safety of enrofloxacin for Carassius auratus gibelio, the acute toxicity of enrofloxacin and its influences on blood biochemical indicators were studied, based on the conventional dose of enrofloxacin in aquaculture. The results showed that the median lethal dose and safe dose of enrofloxacin for C. auratus gibelio were 1 949.84 rng/kg and 194.98 mg/kg, respectively. Compared with the control group, the content of serum total protein （TP）, glutamic-pyruvic transaminase （ ALT）, aspartate aminotransferase （ AST）, and γ- glutamine transferase （γ-GT） were not significantly changed with the continuous oral administration at 20 mg/ kg as a conventional dose for 30 days, while after the continuous oral administration at 40 mg/kg, 80 mg/kg,160 mg/kg, 320 mg/kg for 30 days, the serum AST content was significantly increased by 23% , 30% , 46% , 86% （P 〈0.05） , and the ALT content was significantly increased by 37% , 42% , 69% , 86% （P 〈0. 05） , respectively. This study determined the safe dose of enrofloxacin and its administration dose range causing liver dysfunction for C. auratus gibelio, and provided a scientific basis for the safe use of enrofloxacin in C. auratus gibelio. Enrofloxacin at 20 mg/kg as a conventional dose does not result in liver dysfunction in C. auratus gibelio.
出处
《动物学杂志》
CAS
CSCD
北大核心
2013年第3期446-450,共5页
Chinese Journal of Zoology
基金
上海海洋大学研究生科研基金项目
科技部863计划项目(No.2011AA10A216)
公益性行业(农业)科技专项资金项目(No.201203085)
上海高校知识服务平台项目(No.ZF1206)
关键词
恩诺沙星
异育银鲫
急性毒性
血液生化指标
Enrofloxacin
Carassius auratus gibelio
Acute toxicity
Blood biochemical indicators
