摘要
以黑曲霉P9为出发菌株,采用紫外高温诱变方法对其进行诱变,以获得耐高温高产葡萄糖酸的黑曲霉菌株。并通过摇瓶发酵试验,对诱变菌株的遗传稳定性进行了研究。结果表明:诱变得到一变异菌株UT-8,其高温发酵产葡萄糖酸钙比普通菌种提高37.42%、酶活性提高33.42%;经连续5次传代,UT-8的发酵生产葡萄糖酸钙性能未出现较大的差异,表现出良好的遗传稳定性。为葡萄糖酸生产工业提供理论基础和技术支持。
Aspergillus niger P9 were taken as the original strain, they were mutagenesised by UV mutation and high temperature to obtain high gluconic acid producing strains. Then the genetic stability of the mutant strain was tested by shake flask fermentation experiments. The results showed that： a mutant strain UT-8 is obtained, it can produce 37.42% more calcium gluconate in high temperature fermentation than ordinary bacteria, and its enzyme activity increased by 33.42%. Altered passaged five times, the fermentative production of calcium UT-8 performance does not appear to a greater difference, it exhibits good genetic stability. It may provide a theoretical and techniicalbasis for gluconic acid production.
出处
《食品工业》
北大核心
2013年第6期91-93,共3页
The Food Industry
关键词
黑曲霉
高温发酵
诱变
葡萄糖酸钙
Aspergillus niger
high temperature fermentation
mutagenesis
calcium gluconate
