摘要
优化肝水解肽体外生物学活性检测方法。调整L02细胞浓度到8×104个/ml,以100 l每孔接种于96孔板中,37℃、5%CO2条件下贴壁时间为24 h,弃去旧的培养基,加入以不含谷氨酰胺和胎牛血清的RPMI 1640培养基稀释的肝水解肽溶液,作用48 h。优化后的方法相对于原方法显著提高了肝水解肽促进L02细胞生长的量效反应关系,能定量评价肝水解肽体外活性。
The original bioassay method of liver hydrolysates in vitro was optimized. The concentration of L02 cell was adjusted to 8xl04cells/ml, added to microplate at 100 gl per well and incubated at 37℃ and 5% CO2 for 24 h. The old media was discarded, and then added the liver hydrolysates solution diluted by RPMI 1640 without glutamine and fetal bovine serum, and treated for 48 h. The optimized method had significantly improved the dose response relationship of promoting cell proliferation with respect to the original method, which can be used in quantitative analysis of bioactivity.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2013年第6期600-603,共4页
Chinese Journal of Pharmaceuticals
关键词
L02细胞
肝水解肽
生物学活性
测定
优化
L02 cell
liver hydrolysate
bioactivity
determination
optimization
