摘要
目的从手术切除的人胃肠道问质瘤组织中建立间质瘤细胞株，并探讨其生物学特性。方法将胃肠间质瘤组织切成小块，置于含20％胎牛血清（FBS）的RPMI1640培养基中进行培养，待细胞达80％-90％融合后消化传代培养，并进行形态学、免疫荧光、免疫组织化学、Westernblot及流式细胞术等生物学特性研究。结果成功建立胃肠道问质瘤（GIST）细胞株，该细胞株可在体外培养生存1个月，传代7—8代。经流式细胞仪的免疫荧光分析显示该细胞：CD117＋CD34＋。且所建立的GIST细胞表达c-kil蛋白，ST1571抑制所建立的GIST细胞增殖。结论间质瘤细胞株可在体外长期培养，且具有明显的间质瘤细胞特征。
Objective To establish the cell lines from specimens of surgically resected human gas- trointestihal stromal tumors （GIST） and to identify their biological characters. Methods The tumor tissues from human GIST were cultured in RPMI 1640 media supplemented with 20% fetal bovine serum. After growing to 80%-90% confluence, the cells were detached for subculture and their characteristics, inclu- ding morphology, immunofluorescence staining, immunohistochemical analysis, Western bloting and flow cytometry were determined. Results GIST cells were successfully established. The cell line was passaged for about 7-8 times in one mouth. Immunofluosescence statining showed CD117 ＋ CD34＋. Moreover, the GIST cell lines expressed c-kit protein, and STI571 inhibited the proliferation of the GIST cell lines. Conclusion The GIST cell line was successfully established in vitro and had specific biological characters of GIST.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第6期1252-1254,共3页
Chinese Journal of Experimental Surgery
基金
湖北省自然科学基金资助项目（2010CDB09202）
关键词
胃肠道间质瘤
细胞培养
生物学特性
Gastrointestihal stromal tumors
Cell culture
Biological characters
