摘要
目的观察γ-突触核蛋白（SNCG）在前列腺癌的表达及恶性进展中的作用。方法应用逆转录-聚合酶链反应（RT—PCR）、Westernblot检测SNCG在前列腺细胞株的表达。组织免疫组织化学检测正常前列腺组织、前列腺炎、良性前列腺增生、高级别前列腺上皮内瘤及激素依赖性前列腺癌组织的SNCG表达。结果SNCG在激素依赖性前列腺癌细胞株CWR22、LNCaP的表达明显高于前列腺上皮细胞株RWPE—γ。SNCG在10例正常前列腺组织（1／10）、10例前列腺炎组织（0／10）及10例良性前列腺增生组织（2／10）几乎不表达；在高级别前列腺上皮内瘤（HGPIN）中低表达（3／10）；在122例激素依赖性前列腺癌组织有74例阳性表达。SNCG在激素依赖性前列腺癌中的阳性率为60．66％（74／122），其高表达与前列腺癌中高危因素[前列腺特异抗原（PSA）≥10μg/L、Gleason评分≥7、临床分期≥Tb组]及淋巴结转移相关。结论SNCG在激素依赖性前列腺癌中的异常表达，可能参与前列腺癌恶性进展。
Objective To study the expression and significance of synnclein-gamma （SNCG） in human prostate cancer. Methods SNCG expression in the prostate epithelial cell line （ RWPE-I ） and the hormone-dependent prostate cancer cell lines （LNCaP, CWR22） was detected by using reverse tran- scriptase-polymerase chain reaction （RT-PCR） and Western blotting. The immunohistochemistry was per- formed to investigate the expression of SNCG in the normal prostate tissue, prostatitis tissue, benign prosta- tic hyperplasia （BPH） tissue, high-grade prostatic intraepithelial neoplasia, and hormone-dependent pros- tate cancer （HDPC） tissue. Results The expression of SNCG in hormone-dependent prostate cancer cell line was significantly higher than that in prostate epithelial cell line. Almost no SNCG was detectable in be- nign prostate specimens （10 normal prostate tissues, 10 prostatitis tissues, and 10 BPH tissues）, SNCG was partially expressed in high-grade prostatic intraepithelial neoplasia （3/10） , and the expression was el- evated to a high level in HDPC tissues （the positive rate 60. 66%, 74/122）. Combined with the SNCG stain score and the clinicopa, hological parameters of the corrresponding PCa patients, we found that SNCG protein expression was associated with middle-high-risk PCa characterized by prostate specific antigen （PSA） ≥10μg/L, Gleason score ≥7, and clinical stage≥T2b, and correlated with lymph node metastasis. Conclusion These resuhs suggested that abnormal expression of SNCG may be involved in malignant progression of PCa.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第6期1184-1186,共3页
Chinese Journal of Experimental Surgery
基金
基金项目：福建省自然科学基金资助项目（2012J05140）
