摘要
为了探讨猪股骨头胶原蛋白水解产物对自发性高血压大鼠(SHR)血压的影响,先后进行单次灌胃和连续灌胃实验。先将SHR随机分为8组,每组8只:阴性对照组灌胃蒸馏水,A、B、C、D、E、F实验组分别按照20、30、40、50、60、70mg/kg剂量灌胃猪股骨头原蛋白水解产物,阳性对照组灌胃10mg/kg卡托普利,灌胃后每2h采用尾动脉无创测压法分别测定各组SHR尾动脉收缩压。选取降压效果较好的3个胶原蛋白水解产物实验组作为连续灌胃的实验组,同时设阴性对照组和阳性对照组,分别灌胃蒸馏水和10mg/(kg·d)卡托普利,每组8只,每天灌胃,连续灌胃30d。每5d测量收缩压(SBP)和舒张压(DBP)的变化。结果表明:与阴性对照组相比,B、D、F剂量组降压效果较优,选为连续灌胃的低、中、高剂量组;连续灌胃高、中、低剂量猪股骨胶原蛋白水解产物均可极显著(P<0.01)的降低SHR的SBP和DBP。猪股骨头胶原蛋白水解产物能够显著降低SHR的血压。
To investigate the effect of collagen hydrolysates from swine femur head on the blood pressure of spontaneous hypertension rats （SHRs）, 64 SHRs were randomly divided into 8 groups with 8 rats in each group. Six of these groups were administrated with 20, 30, 40, 50, 60 mg/kg and 70 mg/kg body weight of the hydrolysates. Negative control was orally administrated with the same volume of vehicle （distilled water） and positive control was administrated with 10 mg/kg body weight of captopril. After administration, the systolic pressure of rats was measured every two hours to determine the anti- hypertension effectiveness of collagen hydrolysates. The results obtained showed that oral administration of the hydrolysates at doses of 30, 50 mg/kg and 70 mg/kg body weight significantly lowered the systolic pressure of SHRs, which were then used as low, medium and high doses of hydrolysates for further study. In the next study, 8 rats in each group were then administrated with 30, 50 mg/（kg·d） and 70 mg/（kg·d） of the collagen hydrolysates or equal volume of distilled water （negative control） or 10 mg/（kg·d） of captopril （positive control） for 30 days. The systolic pressure and diastolic pressure of rats were measured every 5 days. The results showed that, compared to negative control, all three doses of hydrolysates significantly decreased both systolic and diastolic pressure of rats （P 〈 0.01）, showing its anti-hypertension property which might be used for human health care.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第11期285-287,共3页
Food Science
关键词
猪股骨头
胶原蛋白水解物
血压
swine femur head
collagen hydrolysates
blood pressure
