摘要
[目的]检测细胞因子基质金属蛋白酶(matrix metalloproteinases,MMPs)MMP-3,IgG和CD68在青少年与中老年患者突出腰椎间盘组织中的表达,探讨两个年龄段腰椎间盘突出的病因。[方法]收集42例腰椎间盘突出症患者的突出腰椎间盘髓核标本,青少年组18例;年龄11～25岁,平均(20.59±3.39)岁;中老年组24例,年龄30～72岁,平均(47±11.15)岁。HE染色观察标本退变情况,免疫组化检测MMP-3,IgG和CD68的表达,光学显微镜下观测并记录数据。[结果]MMP-3阳性率青少年组(33.3%)低于中老年组(79.2%);IgG阳性率青少年组(66.7%)高于中老年组(29.2%);CD-68阳性率青少年组(83.3%)高于中老年组(54.2%),两组MMP-3,IgG和CD68的表达阳性率比较,均P<0.05,差异均有统计学意义。标本HE染色提示中老年组腰椎间盘存在明显退变,青少年组椎间盘退变不明显或无退变。[结论]病理表明中老年组突出腰椎间盘组织存在明显退变,而青少年组退变不明显。免疫组化检测显示中老年组MMP-3明显高于青少年组,但青少年组IgG和CD68均明显高于中老年组,免疫和炎症反应可能是青少年腰椎间盘突出的重要病因,而中老年腰椎间盘突出可能主要与退变有关。
[ Objective ] To determine the expression of cytokines： （matrix metalloproteinases, MMPs） MMP- 3, IgG and CD68, in the herniated tissues of the young and the senior . And to explore the pathogenesis of lumbar intervertebral disc herniation at the two ages. [ Methods] Disc samples from 42 patients with lumbar intervertebral disc herniation were collected. The patients were grouped as the young group , 18 patients , with the avereage age of （20. 59±3.39） years; and the older group, 24 patients, with the avereage age of 47±11.15 years. Degeneration in the intervertebral disc was detected by HE sla- ing. The expression of MMP - 3, IgG and CD68 was detected by immunohistochemistry. Then data were collected and counted by optical microscope. [ Results] There were less samples with MMP -3 in young group （33.3%） than older group （79. 2% ） ; and samples with IgG in young group （66. 7% ） were more than older group （29. 2% ） ; while cases with CD68 in young group （83.3%） were more than older group （54. 2% ） . Positive expression of MMP -3, IgG and CD68 in two group were not the same, and there were significant difference between the two group. Discs of the senior group were proved of degen- erated tissue by HE slaing, while the young group were not seriously degenerated as the older, some even without degeneration. [ Conclusion ] Pathological proved the older group herniated lumbar discs were degenerated tissue, but the young group were not obviously degenerated as the older were. Immunohistochemistry made clear that the older group was with higher rate of positive expression of MMP - 3 than the young group, but expression of IgG and CD68 in the young group were both significantly higher than the older. Immune and inflammation may be the main reason of disc herniation in the young, and lumbar disc herniation in the older may mainly due to disc degeneration.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2013年第11期1121-1126,1132,共7页
Orthopedic Journal of China
关键词
腰椎间盘突出
细胞因子
免疫组化
青少年
lumbar intervertebral disc herniation, eytokines, immunohistochemistry, youngsters
