摘要
目的探讨脑源性神经营养因子(BDNF)和神经营养因子3(NT-3)在扬子鳄大脑胚后发育中的时序表达。方法用免疫组织化学染色和Western blotting法观察BDNF和NT-3在0岁、1岁和2岁的扬子鳄大脑皮质中的定位和含量变化。结果免疫组织化学显示,0岁扬子鳄大脑皮质区内4层均可见BDNF阳性细胞,随着年龄增加,BDNF阳性细胞数量逐渐增多,到2岁时BDNF阳性细胞胞体增大,突起增多,主要集中在细胞层和外网状层移行区。这与Western blotting检测结果一致。0岁扬子鳄大脑皮质区内可见NT-3阳性细胞,主要集中在细胞层和外网状层。到1岁时,NT-3阳性细胞逐渐增多,在外网状层可见大量密集的阳性细胞。2岁时,外侧的分子层中NT-3阳性细胞增多,且细胞突起随着年龄增加而出现增多。这一结果与Western blotting检测一致。结论 BDNF和NT-3参与了扬子鳄胚后2年内大脑皮质区神经细胞的生长发育。
Objective To investigate sequential changes of brain-derived neurotrophic factor （BDNF） and ncurotrophin-3 （NT-3） expressed in the post-embryonic development in cerebral cortex of Alligator sinensis. Methods Immunohisto chemistry technique and Western blotting assay were used to detect the temporal expression concerning its variation at location and content of BDNF and NT-3 in the cerebral cortex of the Chinese alligator by postembryonic age of 0 year（newly hatched） , 1 year and 2 years. Results Immunohistocbemieal test showed that BDNF-positive cells were seen at the 4 layers of cerebral cortex from the alligator aged 0 and increased with ages. By 2 years, the cell body for BDNF- positive cells gradually expanded, with the increased nerve processes that were mostly observed at the transition zone of the cell layer and the outer plexiform layer, which was consistent with Western blotting findings. NT3-positive cells were also seen in cerebral cortex of the newly hatched alligator, concentrated in the cell layer and the outer plexiform layer, and gradually increased by 1 year of age, with more intensive positive cells in the outer plexiform layer. By 2 years, NT3- positive cells increased in the molecular layer at lateral zone, and similarly, cell projections tended to increase with ages, which agreed with the results by Western blotting assay. Conclusion Both BDNF and NT-3 are involved in the post- embryonic development in cerebral cortex of Chinese alligator within 2 years of age.
出处
《解剖学报》
CAS
CSCD
北大核心
2013年第3期308-312,共5页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(31272337)
关键词
大脑
胚后发育
脑源性神经营养因子
神经营养因子-3
免疫组织化学
免疫印迹法
扬子鳄
Cerebral cortex
Postembryonic development
Brain-derived neurotrophic factor
Neurotrophin-3
Immunohistochemistry
Western blotting
Chinese alligator
