摘要
目的评价瓠子乙醚提取物的体外抗肿瘤作用。方法以噻唑蓝(MTT)比色法检测不同浓度瓠子果肉和瓤乙醚提取物培养的肿瘤细胞OD值,分析其对乳腺癌MDA-MB-231细胞、结肠癌HCT116细胞、白血病CCRF-CEM细胞和肺腺癌A549细胞的体外生长抑制作用。结果瓠子果肉和瓤乙醚提取物对MDA-MB-231和HCT116细胞增殖无明显抑制作用,对CCRF-CEM和A549细胞增殖有一定抑制效果。瓠子瓤乙醚提取物对CCRF-CEM细胞的IC50值为12.23 g/mL,对应丙酮提取物的IC50值为29.05 g/mL;瓠子瓤乙醚提取物对A549细胞的IC50值为83.56 g/mL,对应丙酮提取物的IC50值为75.22 g/mL。结论瓠子乙醚提取物对肿瘤细胞增殖有一定抑制作用,有效成分及作用机制有待进一步研究。
Objective To study the anti-tumor effect of extracts from pericarpium lagenariae hispidae in vitro.Methods The effect in vitro of extracts from pericarpium lagenariae hispidae was detected by MTT assay in several cell lines treated with extracts from pericarpium lagenariae hispidae esposure at different concentrations.Results The growth of human breast cancer MDA-MB-231 and colon cancer HCT116 cells were no significantly inhibited.There was significant effect of extracts from pericarpium lagenariae hispidae for growth inhibitory against leukemia cells CCRF-CEM and lung cancer cells A549.The IC 50 value of its ether extract and acetone extract for growth inhibitory against CCRF-CEM cell line was 12.23 g/mL and 29.05 g/mL respectively,and the IC 50 value against A549 cell line was 83.56 g/mL and 75.22 g/mL respectively.Conclusion The extracts from pericarpium lagenariae hispidae can significantly inhibit growth of tumor cell.
出处
《世界临床药物》
CAS
2013年第5期289-291,301,共4页
World Clinical Drug
关键词
瓠子
肿瘤细胞
MTT比色法
pericarpium lagenariae hispidae
tumor cell
MTT assay
