摘要
目的：建立桑姜感冒胶囊中总黄酮含量的测定方法。方法：采用紫外-可见分光光度法,以芦丁为对照品,以506 nm作为测量波长进行测定。结果：芦丁在0.5~3.0 mg范围内与吸光度呈良好的线性关系（r=0.999 7）,平均回收率为96.63%,RSD为1.91%。结论：该方法操作简便、准确、重现性好,可用于测定桑姜感冒胶囊中总黄酮的含量。
Objective： To establish UV spectrophotometry method for the determination total flavonoids in compounds of Sangjiang Ganmao capsule. Methods： The contents of the total flavonoids were determined at 506 nm with rutin as the standard substance by ultraviolet UV-visible method. Results： The calibration curve was linear in the range of 0.5-3.0 mg for rutin, r=0.999 7; the average recovery was 96.63%, RSD= 1.91% （n=6）. Conclusion： This method is simple, fast and accurate, and can be used as the determination of the total flavonoids in compound Sangjiang Ganmao capsule.
基金
大理学院大学生科研基金资助项目(KYSX2011071)
