摘要
目的 研究临床肝移植过程中离体供肝低温保存 再灌注期间肝实质细胞凋亡及其相关机制。方法 应用细胞凋亡原位末端标记法并结合电镜观察 ,检测低温保存时间分别为 0、3、6、及 9h的 4组兔肝脏在低温保存 再灌注过程中肝细胞凋亡发生情况。同时 ,对 4组肝脏再灌注前、后组织内氧自由基相关指标进行测定。结果 各组肝脏于低温保存后的再灌注期间 ,组织内可见明显的肝实质细胞凋亡现象 ,且低温保存时间越长的肝脏 ,再灌注后凋亡细胞数量越多 ;但各组肝脏于低温保存末 ,组织内则未见或仅偶见肝实质细胞凋亡。此外 ,4组肝脏实质细胞凋亡数量与相应组织内氧自由基含量间呈显著的正相关关系。结论 肝实质细胞凋亡显著参与了肝脏低温保存 再灌注损伤过程 ,组织内氧自由基的过量产生可能是肝实质细胞凋亡的重要机制。
Objective To study hepatocytic apotosis during cold preservation reperfusion of isolated liver and its mechanism during liver transplantation. Methods Four groups of rabbit livers, which had been preserved in cold storage for 0、3、6、9 h respectively, were observed during reperfusion with TdT mediated dUTP biotion nickend labeling and electron microscope. Besides, SOD, GSH and MDA in liver tissue were observed at the time points before and after reperfusion. Results Apoptotic hepatocyces were obviously observed in reperfusec livers after cold storage. Furthermore, the longer the cold storage, the greater the number of apoptotic cells. On the contrary, no or a few apoptotic hepatocytes appeared in all the livers preserved in low temperature. In addition, there was significant positive correlation between the numbers of apoptotic hepatocytes and contents of oxygen free radicals. Conclusion Apoptosis of hepatocyts occurred markedly in the donor liver after cold preservation reperfusion, and increased oxygen free radicals may play a rale to induce hepatocytic apoptosis .
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2000年第9期868-871,共4页
Journal of Third Military Medical University
关键词
肝脏
再灌注损伤
氧自由基
细胞凋亡
低温保存
liver
preservation reperfusion injury
apoptosis
oxygen free radicals
rabbit
