摘要
以抗病资源收集一号和感病农家品种皇后杂交的F1、BCs、BCr、F2以及双亲为材料,苗期进行白粉病抗性鉴定,分析了白粉病抗源收集一号的抗性遗传规律,并利用集团分离分析法结合SRAP标记进行连锁分析。结果表明,收集一号对白粉病Podosphaera xanthii生理小种1的抗性由显性单基因控制,同时对1 600对SRAP引物进行筛选,引物me4em37扩增出的多态性条带与抗病基因表现连锁关系,该多态性片段大小为100 bp,与抗病基因遗传连锁距离为17.0 cM。
Parental lines and（No.1Shouji×Huanghou）F1,F2 and backcross populations were scored for response to inoculation with Podosphaera xanthiirace 1.The sequence related amplified polymorphism（SRAP）technique and bulk segregant analysis（BSA）were used to identify molecular markers linked to the resistance of melon powdery mildew.The resistance to P.xanthii race 1 in No.1 Shouji was controlled by one single dominant gene.A total 1 600 pairs SRAP primers could detect the polymorphism between the resistant pool and susceptible pool.An unique 100 bp fragment was amplified with the primer me4em37.It was identified that is linked to resistance gene of No.1 Shouji at a distance of 17.0 cM.
出处
《华北农学报》
CSCD
北大核心
2013年第2期73-77,共5页
Acta Agriculturae Boreali-Sinica
基金
国家西甜瓜产业技术体系项目(CARS-26-04)
新疆自治区高技术研究与发展项目(201111119)
关键词
甜瓜
白粉病
抗病基因
SRAP分子标记
Cucumis melo L.
Powdery mildew
Disease resistance gene
SRAP marker
