摘要
采用碳化二亚胺法将三聚氰胺偶联到牛血清白蛋白上合成免疫原三聚氰胺-牛血清白蛋白。合成的抗原进行紫外扫描鉴定,利用辣根过氧化物酶合成HRP-MEL-BSA酶标抗原,利用固相抗体酶标抗原来进行效价的测定,检测抗原的合成。
Through the method of EDC, the melamine (MEL)was coupled to the carrier protein for synthesizing the artificial antigen,Artificial antigen was identified by UV,Use horseradish peroxidase (HRP) to combine with MEL-BSA in order to produce enzyme labeled antigen.Using coated antigen enzyme labeled antigen to detected MEL-BSA antigen.
出处
《安徽农学通报》
2013年第10期19-21,共3页
Anhui Agricultural Science Bulletin
基金
厦门市重大科技计划项目3502Z20081002
3502Z20091004