摘要
利用棉花EST序列数据库开发EST-SSR标记,对108 558条棉花EST序列进行SSR搜索,针对286条含有SSR的EST序列设计合成了107对引物,选用12个棉花品种进行有效性检测。结果表明,在陆地棉EST-SSR中,二核苷酸和三核苷酸占主导地位,所占比例分别为32.0%和53.6%;出现最多的重复基元是AT/TA,占总数的19.6%;在海岛棉EST-SSR中,三核苷酸和六核苷酸占主导地位,所占比例分别为36.6%和36.8%;出现最多的重复基元是AT/TA,占总数的7.4%。PCR检测表明,93个引物对(86.9%)可以扩增出稳定清晰条带458条,平均每对引物检测到4.93个位点。利用93对多态性引物,初步对12份棉花种质资源进行遗传多样性分析,将供试材料按亲缘关系远近分为3大类,来源相同的品种大致聚在一类,呈现出一定的地域性分布规律。分析结果表明,从棉花相关EST数据库中开发的SSR引物有较好的实用性。
The objective of this study was to develop new simple sequence repeat （SSR） markers from EST （Expressed sequence tag） sequences （EST-SSR markers） for cotton. 108558 EST sequences of Gossypiurn were used to screen SSR.Based on the 286 SSR-containing ESTs,a total of 107 of pairs primer were successfully designed.Whose validity were detected using 12 cotton varieties.The results showed that the dinucleotides and tricleotides gained advantage in the upland cotton EST-SSR, whose proportion was 32.0% and 53.6% respectively.The AT/TA EST-SSRs were 36.6% and 36.8% ;The total of AT/TA was 7.4%.The PCR test showed that 93 primer （86.9%） may amplify 458 stealy and clear bands,the total of 4.93 locus were detected from each pair of primers.The total of 12 sets of cotton germplasm resources were initially analyzed with genetic heredity diversity,whose tested materials were divided into 3 groups by phy- logenetic relationships.Each group was included in the varieties with roughly similar parents or similar re- geonal distribution.The result showed that the developed SSRs primers from the related ESTs data bank of gossypium were more practicable.
出处
《新疆农业大学学报》
CAS
2013年第2期118-123,共6页
Journal of Xinjiang Agricultural University
基金
新疆维吾尔自治区自然科学基金项目(2010211A16)
国家自然科学基金项目(31060151)
