摘要
目的 探讨药物控制释放载体与组织工程载体的制备方法与结构形态。方法 用新型生物可降解材料聚羟基丁酸酯-羟基戊酸酯共聚物[poly(hydroxybutyrate-hydroxyvalerate) PHBV]、聚己内酯[poly(ε-caprolatone), PCL]及其共混物为基材，用乳化-溶剂蒸发法制备不同结构形态微球。结果 平均粒径为30.5μm，粒径分布较窄（跨距SPAN=1.18），呈正态曲线分布。扫描电镜观察, PHBV微球表面呈不规则多孔皱缩结构形态; PCL微球表面光滑，无孔洞；PCL/PHBV共混微球呈有规则多孔洞形态结构，随着基材中PCL成分增加，微球孔洞大小与数目也随之增加。结论 通过选择不同生物材料及共混方法，改变微球的结构形态，以满足不同药物释放系统与组织工程对载体性能与形态的不同要求。
Objective To explore the preparation methods and structural morphology of the carries for drug-controlled release and the tissue engineering. Methods Microspheres (MS) with different structural morphologies were prepared using the emulsion-solvent evaporation technique based on new biodegradable materials poly(3-hydroxybutyrate-hydroxyvalerate) (PHBV), poly(ε-caprolactone)(PCL), and their blend. Results The mean diameter of MS was 30.5 μm, The narrow size range was determined (SPAN=1.18). In PHBV microspheres shriveled appearance and irregular micropori on the surface were observed under scanning electron microscope. PCL microspheres had intact wall with a fairly smooth surface, essentially without pores. However, the microspheres with different blend ratios of PCL to PHBV had surface regularly perforated by numerous discrete micropores. The size and number of the pores increased with the proportion of PCL. Conclusion The structural morphology of microspherers could be changed via selecting different biomaterials and using blend methods to offer interesting possibilities for some special purpose of bioactive macromolecules and tissue engineering.
出处
《第一军医大学学报》
CSCD
2000年第4期336-338,共3页
Journal of First Military Medical University
基金
国家自然科学基金资助项目(39970223
39970876)
军队医药科研基金资助项目(98M065)
关键词
聚羟基丁酸酯
羟基戊酸酯共聚物
聚己内酯
微球
hydroxybutyrate
hydroxyvalerate
caprolactone
structural morphology
microspheres
