摘要
为研究乳酸菌体外抗病毒作用,在鸡传染性支气管炎病毒(IBV)感染的鸡胚成纤维细胞(CEF)模型上,分析比较了6株乳酸菌对细胞内两种抗氧化酶:超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性的影响,以及丙二醛(MDA)含量的变化,结果显示,与病毒感染对照组相比,6株乳酸菌均显著提高了细胞总抗氧化能力(P<0.01)、显著降低了MDA含量(P<0.01)。研究结果表明,乳酸菌能促进细胞在病毒感染状态下产生更强的抗氧化能力来清除自由基,并减少自由基所引起的氧化损伤。
In order to study the antiviral activity of Lactobacillus in vitro, we compared the effects of 6 strains of Lactobaeillus on the contents of two anti-oxidase （SOD and GSH-Px ）and MDA in CEF by two methods. The first one was to add the Lactobacillus culture supernatant to the CEF first, and then infected by IBV. The second one was to add the LactobaciUus culture supernatant to the CEF and infected by IBV at the same time. The result showed that the anti-oxidant activity of CEF had increased significantly （P〈0.01） in both two methods. The con-tents of MDA had reduced significantly （P〈0.01）. The results showed that, Lactobacillus could promote the an-tioxidant activity of virus-infected cells to clean free radicals and reduce their oxidative damage.
出处
《中国兽医杂志》
CAS
北大核心
2013年第1期27-29,共3页
Chinese Journal of Veterinary Medicine
关键词
乳酸菌
CEF
抗病毒
抗氧化
Lactobacillus
CEF
anti-virus
anti-oxidant
