摘要
目的:重新探讨定义乙肝表面抗原酶联免疫吸附试验(ELISA)试剂盒的检测"灰区"。方法 :以乙肝表面抗原定值质控血清为标本对ELISA和电化学发光(ECLIA)两种检测方法进行灵敏度比较试验;同时用ELISA试剂盒检测由ECLIA检测为乙肝表面抗原弱反应性的标本,从而重新考虑ELISA试验时的复查"灰区"设定。结果:ELISA检测灵敏度为0.5 IU/mL,ECLIA检测灵敏度为﹤0.125 IU/mL;经过筛选标本后,用ELISA试剂盒检测由ECLIA检测为乙肝表面抗原弱反应性的标本的S/CO值为0.24～1。结论:ECLIA的检测灵敏度高于ELISA;同时为了防止漏检,目前ELISA检测HBsAg的所使用的复查"灰区"有必要进一步扩大。
Objective To discuss the grey zone of the detection for HBsAg by ELISA kit.Methods Fixed value quality control sera were included as samples,and the sensitivities of ELISA kit and ECLIA kit for detecting HBsAg were compared,and then the samples which were weak active testified by ECLIA kit were retested by ELISA kit to discuss the grey zone of the specimens for rechecking.Results The sensitivity of ELISA kit and ECLIA kit were 0.5 IU / mL and less than 0.125 IU / mL,respectively,and after screening,the S / CO of the samples defined as weak active by ECLIA kit and retested by ELISA kit was 0.24-1.Conclusion The sensitivity of ECLIA was better than that of ELISA.For avoiding false negative results,the grey zone for rechecking of ELISA kit should be enlarged.
出处
《实用医学杂志》
CAS
北大核心
2013年第10期1679-1682,共4页
The Journal of Practical Medicine
