摘要
目的:研究桂枝芍药知母汤对Ⅱ型胶原蛋白诱导类风湿关节炎大鼠踝关节骨组织细胞RANKL表达的影响,以证明桂枝芍药知母汤可通过抑制破骨细胞的分化与活化,达到缓解或阻止关节的损伤,已达到对类风湿关节炎骨破坏的抑制作用。方法 :采用Ⅱ型胶原蛋白诱导类风湿关节炎大鼠模型,灌胃给药30 d后用原位杂交技术检测踝关节局部的OPG/RANKL水平。结果 :桂枝芍药知母汤能降低CIA踝关节组织RANKL、OPG表达水平。结论 :桂枝芍药知母汤可以抑制CIA模型鼠关节RANKLmRNA的表达水平,提高OPGmRNA的表达水平,降低RANKL/OPG的比值,可能通过抑制了破骨细胞的分化与活化,从而可能缓解或阻止关节的损伤破坏。
Objective ： To study the influence of Guishao Zhimu Decoction （ GSZ ） on the CIA, to see what happens to the RANKL of the angle joint of CIA.So it will be proved that GSZ is supposed to restraint the damage of RA. Methods ： Type II collagen protein was used to induce the CIA model.30 d after administration of （;SZ, change of OPG/ RANKL in the part of its angle joint was tested with ISH. Result： GSZ can lower the level of RANKL/OPG. Conclusion ： This method can restrain the damage of RANKL towards angle joint.Through restraining the differentiation and activation of osteoclast, joint injury can be relieved, and even he prevented.
出处
《辽宁中医药大学学报》
CAS
2013年第6期24-25,共2页
Journal of Liaoning University of Traditional Chinese Medicine
基金
辽宁省自然科学基金资助项目(201202262)
