摘要
目的:研究高脂饮食对C57BL/6J小鼠肝脏组织糖异生的影响。方法:将8周龄雄性C57BL/6J小鼠随机分为普通饮食组和高脂饮食组。饲养14周后处死,收集血清和肝脏组织,ELISA和酶学方法检测血清与肝脏组织中甘油三脂(triglyceride,TG)、游离脂肪酸(free fatty acid,FFA)含量,测定血清中胰岛素水平;过碘酸-希夫(periodic acid-Schiff,PAS)染色观察肝细胞糖原颗粒;实时定量PCR和Western blot分别检测肝脏组织糖异生相关的磷酸烯醇式丙酮酸羧激酶(phosphoenolpyruvate car-boxykinase,PEPCK)、葡萄糖-6-磷酸激酶(glucose-6-phosphatase,G-6-Pase)的基因和蛋白表达水平;葡萄糖耐量实验评价小鼠胰岛素抵抗程度。结果:饲养14周后,高脂饮食组小鼠体质量较普通饮食组明显增加;与普通饮食组相比,高脂饮食组小鼠血清与肝脏组织中TG、FFA含量明显增加;PAS染色表明高脂饮食组肝脏糖原含量明显增多;基因和蛋白检测结果显示高脂饮食组肝脏组织中糖异生相关的基因、蛋白表达增加;同时空腹血糖及血清胰岛素明显增加;葡萄糖耐量实验异常,血糖峰值延迟。结论:高脂饮食使C57BL/6J小鼠肝脏组织PEPCK、G-6-Pase表达增加,糖异生增加,导致空腹血糖增加,葡萄糖耐量受损。
Objective：To investigate the effects of high fat diet on gluconeogenesis in hepatic tissues of C57BL/6J mice.Methods：Male C57BL/6J mice aged 8 weeks were randomly assigned with high fat diet or normal chow diet.Serum free fatty acid（FFA）,triglyceride（TG） and insulin levels in serum and hepatic tissues were determined by enzymatic methods and ELISA kits.Glycogen granule was evaluated by periodic acid-Schiff（PAS） staining.mRNA and protein levels of hepatic phosphoenolpyruvate carboxykinase（PEPCK） and glucose-6-phosphatase（G-6-Pase） were measured by real-time PCR and Western blot.Glucose tolerance test was used to evaluate body insulin sensitivities.Results：Weight was higher in high fat diet mice than in normal chow diet mice.High fat diet elevated levels of TG and FFA in C57 mice.PAS staining revealed an increase of glycogen granule.mRNA and protein expressions of G-6Pase and PEPCK were increased in high fat diet mice.High fat diet also increased the fasting glucose levels and induced glucose resistance in C57 mice.Conclusions：High fat diet upragulates expressions of PEPCK and G-6-Pase,promotes gluconeogenesis,resulting in high fasting blood-glucose.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2013年第4期337-340,共4页
Journal of Chongqing Medical University
基金
国家自然科学基金资助项目(编号:81070631、81270493)
关键词
高脂饮食
肝脏组织
脂质积聚
糖异生
high fat diet
hepatic tissues
lipid deposition
gluconeogenesis
